Please use this identifier to cite or link to this item: https://hdl.handle.net/1822/54197

TitlePortrait of matrix gene expression in Candida glabrata biofilms with stress induced by different drugs
Author(s)Rodrigues, Célia F.
Henriques, Mariana
KeywordsCandida
Biofilm
Candida glabrata
Genes
Resistance
Antifungal
Matrix
Biofilms
Drug resistance
Gene expression
Issue dateApr-2018
PublisherMultidisciplinary Digital Publishing Institute (MDPI)
JournalGenes
CitationRodrigues, Célia F.; Henriques, Mariana, Portrait of matrix gene expression in Candida glabrata biofilms with stress induced by different drugs. Genes, 9(205), 1-16, 2018
Abstract(s)(1) Background: Candida glabrata is one of the most significant Candida species associated with severe cases of candidiasis. Biofilm formation is an important feature, closely associated with antifungal resistance, involving alterations of gene expression or mutations, which can result in the failure of antifungal treatments. Hence, the main goal of this work was to evaluate the role of a set of genes, associated with matrix production, in the resistance of C. glabrata biofilms to antifungal drugs. (2) Methods: the determination of the expression of BGL2, XOG1, FKS1, FKS2, GAS2, KNH1, UGP1, and MNN2 genes in 48-h biofilms cells of three C. glabrata strains was performed through quantitative real-time PCR (RT-qPCR), after contact with Fluconazole (Flu), Amphotericin B (AmB), Caspofungin (Csf), or Micafungin (Mcf). (3) Results: Mcf induced a general overexpression of the selected genes. It was verified that the genes related to the production of -1,3-glucans (BGL2, XOG1, GAS2) had the highest expressions. (4) Conclusion: though -1,6-glucans and mannans are an essential part of the cell and biofilm matrix, C. glabrata biofilm cells seem to contribute more to the replacement of -1,3-glucans. Thus, these biopolymers seem to have a greater impact on the biofilm matrix composition and, consequently, a role in the biofilm resistance to antifungal drugs.
TypeArticle
URIhttps://hdl.handle.net/1822/54197
DOI10.3390/genes9040205
ISSN2073-4425
Peer-Reviewedyes
AccessOpen access
Appears in Collections:CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series

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