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|Title:||Stem cell-containing hyaluronic acid-based spongy hydrogels for integrated diabetic wound healing|
|Author(s):||Silva, Lucília Pereira|
Santos, T. C.
Rodrigues, Daniel Barreira
Pirraco, Rogério P.
Cerqueira, Mariana Teixeira
Reis, R. L.
Correlo, V. M.
Marques, A. P.
|Keywords:||Diabetic foot ulcerations|
|Journal:||Journal of Investigative Dermatology|
|Citation:||da Silva L. P., Santos T. C., Rodrigues D. B., Pirraco R. P., Cerqueira M. T., Reis R. L., Correlo V. M., Marques A. P. Stem Cell-Containing Hyaluronic Acid-Based Spongy Hydrogels for Integrated Diabetic Wound Healing, Journal Of Investigative Dermatology, Vol. 137, pp. 1541-1551, doi:10.1016/j.jid.2017.02.976, 2017|
|Abstract(s):||The detailed pathophysiology of diabetic foot ulcers is yet to be established and improved treatments are still required. We propose a strategy that directs inflammation, neovascularization, and neoinnervation of diabetic wounds. Aiming to potentiate a relevant secretome for nerve regeneration, stem cells were precultured in hyaluronic acid-based spongy hydrogels under neurogenic/standard media before transplantation into diabetic mice full-thickness wounds. Acellular spongy hydrogels and empty wounds were used as controls. Reepithelialization was attained 4 weeks after transplantation independently of the test groups, whereas a thicker and more differentiated epidermis was observed for the cellular spongy hydrogels. A switch from the inflammatory to the proliferative phase of wound healing was revealed for all the experimental groups 2 weeks after injury, but a significantly higher M2(CD163 Ã¾ )/M1(CD86 Ã¾ ) subtype ratio was observed in the neurogenic preconditioned group that also failed to promote neoinnervation. A higher number of intraepidermal nerve fibers were observed for the unconditioned group probably due to a more controlled transition from the inflammatory to the proliferative phase. Overall, stem cell-containing spongy hydrogels represent a promising approach to enhance diabetic wound healing by positively impacting re-epithelialization and by modulating the inflammatory response to promote a successful neoinnervation.|
|Appears in Collections:||3B’s - Artigos em revistas/Papers in scientific journals|