Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/51751
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Campo DC | Valor | Idioma |
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dc.contributor.author | Pereira, Filipa Alexandra Barroso | por |
dc.contributor.author | Azevedo, Flávio Humberto Torres Dias Feio | por |
dc.contributor.author | Parachin, Nadia Skorupa | por |
dc.contributor.author | Hahn-Hagerdal, Barbel | por |
dc.contributor.author | Gorwa-Grauslund, Marie F. | por |
dc.contributor.author | Johansson, Björn | por |
dc.date.accessioned | 2018-03-07T17:14:58Z | - |
dc.date.issued | 2016-05-01 | - |
dc.date.submitted | 2015-11 | - |
dc.identifier.citation | Pereira, F., Azevedo, F., Parachin, N. S., Hahn-Hägerdal, B., Gorwa-Grauslund, M. F., & Johansson, B. (2016). Yeast pathway kit: a method for metabolic pathway assembly with automatically simulated executable documentation. ACS synthetic biology, 5(5), 386-394 | por |
dc.identifier.issn | 2161-5063 | por |
dc.identifier.uri | https://hdl.handle.net/1822/51751 | - |
dc.description.abstract | We have developed the Yeast Pathway Kit (YPK) for rational and random metabolic pathway assembly in Saccharomyces cerevisiae using reusable and redistributable genetic elements. Genetic elements are cloned in a suicide vector in a rapid process that omits PCR product purification. Single-gene expression cassettes are assembled in vivo using genetic elements that are both promoters and terminators (TP). Cassettes sharing genetic elements are assembled by recombination into multigene pathways. A wide selection of prefabricated TP elements makes assembly both rapid and inexpensive. An innovative software tool automatically produces detailed self-contained executable documentation in the form of pydna code in the narrative Jupyter notebook format to facilitate planning and sharing YPK projects. A D-xylose catabolic pathway was created using YPK with four or eight genes that resulted in one of the highest growth rates reported on D-xylose (0.18 h(-1)) for recombinant S. cerevisiae without adaptation. The two-step assembly of single-gene expression cassettes into multigene pathways may improve the yield of correct pathways at the cost of adding overall complexity, which is offset by the supplied software tool. | por |
dc.description.sponsorship | - This work was supported by the Fundacao para a Ciencia e Tecnologia Portugal (FCT) through Project MycoFat PTDC/AAC-AMB/120940/2010. F.A. was supported by FCT fellowship SFRH/BD/80934/2011. CBMA was supported by the strategic programme UID/BIA/04050/2013 (POCI-01-0145-FEDER-007569) funded by national funds through the FCT I.P. and by the ERDF through the COMPETE2020 - Programa Operational Competitividade e Internacionalizacao (POCI). The authors wish to thank to Dr. Paula Goncalves for the pGXF1 vector, Dr. Daniel Schlieper for the pCAP<SUP>s</SUP> vector, Dr. Yukio Nagano for the pSU0 vector, Dr. Peter Kotter, University of Frankfurt, Germany, for the S. cereivise CEN.PK strains, and Dr. Nina Q Meinander for the p4** vectors. | por |
dc.language.iso | eng | por |
dc.publisher | ACS | por |
dc.rights | closedAccess | por |
dc.subject | Metabolic engineering | por |
dc.subject | Saccharomyces cerevisiae | por |
dc.subject | D-xylose | por |
dc.subject | Synthetic biology | por |
dc.subject | Bioinformatics | por |
dc.title | Yeast Pathway Kit: A Method for Metabolic Pathway Assembly with Automatically Simulated Executable Documentation | por |
dc.type | article | - |
dc.peerreviewed | yes | por |
dc.relation.publisherversion | https://pubs.acs.org/doi/abs/10.1021/acssynbio.5b00250 | por |
oaire.citationStartPage | 386 | por |
oaire.citationEndPage | 394 | por |
oaire.citationIssue | 5 | por |
oaire.citationVolume | 5 | por |
dc.date.updated | 2018-03-07T16:28:51Z | - |
dc.identifier.doi | 10.1021/acssynbio.5b00250 | por |
dc.identifier.pmid | 26916955 | - |
dc.subject.fos | Engenharia e Tecnologia::Biotecnologia Industrial | por |
dc.description.publicationversion | info:eu-repo/semantics/publishedVersion | por |
dc.subject.wos | Science & Technology | - |
sdum.export.identifier | 2945 | - |
sdum.journal | ACS SYNTHETIC BIOLOGY | por |
Aparece nas coleções: | DBio - Artigos/Papers |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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Pereira et al. 2016 b.pdf Acesso restrito! | 1,67 MB | Adobe PDF | Ver/Abrir |