Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/51658
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Campo DC | Valor | Idioma |
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dc.contributor.author | Castro, Pedro Humberto | por |
dc.contributor.author | Verde, Nuno | por |
dc.contributor.author | Lourenço, Tiago | por |
dc.contributor.author | Magalhães, Alexandre Papadopoulos | por |
dc.contributor.author | Tavares, R. M. | por |
dc.contributor.author | Bejarano, Eduardo Rodríguez | por |
dc.contributor.author | Azevedo, Herlânder Anselmo Queirós Pereira | por |
dc.date.accessioned | 2018-03-07T09:28:50Z | - |
dc.date.issued | 2015-12 | - |
dc.identifier.issn | 0032-0781 | por |
dc.identifier.uri | https://hdl.handle.net/1822/51658 | - |
dc.description.abstract | Post-translational modification mechanisms function as switches that mediate the balance between optimum growth and the response to environmental stimuli, by regulating the activity of key proteins. SUMO (small ubiquitin-like modifier) attachment, or sumoylation, is a post-translational modification that is essential for the plant stress response, also modulating hormonal circuits to co-ordinate developmental processes. The Arabidopsis SUMO E3 ligase SAP and Miz 1 (SIZ1) is the major SUMO conjugation enhancer in response to stress, and is implicated in several aspects of plant development. Here we report that known SUMO targets are over-represented in multiple carbohydrate-related proteins, suggesting a functional link between sumoylation and sugar metabolism and signaling in plants. We subsequently observed that SUMO-conjugated proteins accumulate in response to high doses of sugar in a SIZ1-dependent manner, and that the null siz1 mutant displays increased expression of sucrose and starch catabolic genes and shows reduced starch levels. We demonstrated that SIZ1 controls germination time and post-germination growth via osmotic and sugar-dependent signaling, respectively. Glucose was specifically linked to SUMO-sugar interplay, with high levels inducing root growth inhibition and aberrant root hair morphology in siz1. The use of sugar analogs and sugar marker gene expression analysis allowed us to implicate SIZ1 in a signaling pathway dependent on glucose metabolism, probably involving modulation of SNF1-related kinase 1 (SnRK1) activity. | por |
dc.description.sponsorship | This work was funded by the Fundo Europeu De Desenvolvimento Regional (FEDER) [through the Operational Competitiveness Program (COMPETE)], and by Foundation for Science and Technology (FCT) national funds within the scope of project 'SUMOdulator' [FCOMP-01-0124-FEDER-028459 and PTDC/BIA-PLA/3850/2012]; the FCT [grant No. SFRH/BD/44484/2008 to P.H.C.]; European Regional Development Fund (ERDF) [the 'Genomics and Evolutionary Biology' project, cofinanced by North Portugal Regional Operational Program 2007/2013 (ON.2-O Novo Norte), under the National Strategic Reference Framework (NSRF) (to H.A.)]. | por |
dc.language.iso | eng | por |
dc.publisher | Oxford University Press | por |
dc.relation | info:eu-repo/grantAgreement/FCT/5876-PPCDTI/130409/PT | por |
dc.relation | info:eu-repo/grantAgreement/FCT/COMPETE/130409/PT | por |
dc.relation | info:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBD%2F44484%2F2008/PT | por |
dc.rights | closedAccess | por |
dc.subject | Arabidopsis | por |
dc.subject | 2-Deoxyglucose | por |
dc.subject | Glucose | por |
dc.subject | Post-germination growth arrest | por |
dc.subject | SIZ1 | por |
dc.subject | SUMO | por |
dc.title | SIZ1-dependent post-translational modification by SUMO modulates sugar signaling and metabolism in Arabidopsis thaliana | por |
dc.type | article | por |
dc.peerreviewed | yes | por |
oaire.citationStartPage | 2297-311 | por |
oaire.citationEndPage | 2311 | por |
oaire.citationIssue | 12 | por |
oaire.citationVolume | 56 | por |
dc.identifier.doi | 10.1093/pcp/pcv149 | por |
dc.identifier.pmid | 26468507 | por |
dc.description.publicationversion | info:eu-repo/semantics/publishedVersion | por |
dc.subject.wos | Science & Technology | por |
sdum.journal | Plant and Cell Physiology | por |
Aparece nas coleções: | CBFP - Artigos/Papers DBio - Artigos/Papers |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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siz.pdf Acesso restrito! | 2,04 MB | Adobe PDF | Ver/Abrir |