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|Título:||Gellan gum-hydroxyapatite composite spongy-like hydrogels for bone tissue engineering|
|Autor(es):||Manda-Guiba, G. M.|
Silva, Lucília Pereira
Cerqueira, Mariana Teixeira
Pereira, Diana Ribeiro
Oliveira, Mariana Braga
Mano, J. F.
Marques, A. P.
Oliveira, J. M.
Correlo, V. M.
Reis, R. L.
Bone tissue engineering
|Revista:||Journal of Biomedical Materials Research Part A|
|Citação:||Manda-Guiba G. M., da Silva L. P., Cerqueira M. T., Pereira D. R., Oliveira M. B., Mano J. F., Marques A. P., Oliveira J. M., Correlo V. M., Reis R. L. Gellan Gum Hydroxyapatite Composite Hydrogels for Bone Tissue Engineering, J Biomed Mater Res A, Vol. 106, Issue 2, pp. 479–490, doi:10.1002/jbm.a.36248, 2018|
|Resumo(s):||Osteoinductive biomaterials represent a promising approach to advance bone grafting. Despite promising, the combination of sustained biodegradability, mechanical strength, and biocompatibility in a unique biomaterial that can also support cell performance and bone formation in vivo is demanding. Herein, we developed gellan gum (GG)-hydroxyapatite (HAp) spongy-like hydrogels to mimic the organic (GG) and inorganic (HAp) phases of the bone. HAp was successfully introduced within the GG polymeric networks, as determined by FTIR and XRD, without compromising the thermostability of the biomaterials, as showed by TGA. The developed biomaterials showed sustained degradation, high swelling, pore sizes between 200 and 300 Î¼m, high porosity (>90%) and interconnectivity (<60%) that was inversely proportional to the total polymeric amount and to CaCl2Â crosslinker. CaCl2Â and HAp reinforced the mechanical properties of the biomaterials from a storage modulus of 40 KPa to 70-80 KPa. This study also showed that HAp and CaCl2Â favored the bioactivity and that cells were able to adhere and spread within the biomaterials up to 21 days of culture. Overall, the possibility to tailor spongy-like hydrogels properties by including calcium as a crosslinker and by varying the amount of HAp will further contribute to understand how these features influence bone cells performance in vitro and bone formation in vivo.|
|Versão da editora:||https://www.ncbi.nlm.nih.gov/pubmed/28960767|
|Aparece nas coleções:||3B’s - Artigos em revistas/Papers in scientific journals|
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