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https://hdl.handle.net/1822/51187
Título: | Evaluation of the presence of arabinogalactan proteins and pectins during Quercus suber male gametogenesis |
Autor(es): | Costa, Mário Luís Sobral, Rómulo Sacramento Costa, Maria Manuela Ribeiro Amorim, Maria Isabel Coimbra, Sílvia |
Palavras-chave: | Amino acid sequence Epitopes Flowers Molecular sequence data Mucoproteins Organ specificity Pectins Phylogeny Plant Proteins Pollen Quercus Real-time polymerase chain reaction Reverse transcriptase polymerase chain reaction Quercus suber cork oak pollen development arabinogalactan proteins AGP microsporocyte cells walls male gametophyte Arabidopsis thaliana |
Data: | Jan-2015 |
Editora: | Oxford University Press |
Revista: | Annals of Botany |
Resumo(s): | Quercus suber (cork oak) is a dominant tree of the Fagaceae in forests of the south-west Iberian Peninsula. It is monoecious with a long progamic phase that provides a comprehensive system for comparative studies in development and sexual reproduction. In this study the distribution of arabinogalactan protein (AGPs) and pectin epitopes in anthers of Q. suber was assessed to map these hydroxyproline-rich glycoproteins and the galacturonate-rich acidic polysaccharides during pollen development. Methods Immunolocalization in male flowers was performed with a set of monoclonal antibodies directed against the carbohydrate moiety that recognizes AGPs and pectins. To identify AGP genes involved in cork oak male flower development, a search was conducted for annotated AGP genes in the available transcriptome data of the Cork Oak EST Consortium database (www.corkoakdb.org). |
Tipo: | Artigo |
Descrição: | Supplementary data are available online at www.aob.oxford journals.org and consist of the following. Figure S1: Quercus suber predicted AG peptide sequences. Table S1: Gene-specific primers used in qRT-PCR analysis. |
URI: | https://hdl.handle.net/1822/51187 |
DOI: | 10.1093/aob/mcu223 |
ISSN: | 0305-7364 |
e-ISSN: | 1095-8290 |
Arbitragem científica: | yes |
Acesso: | Acesso restrito autor |
Aparece nas coleções: | CBFP - Artigos/Papers DBio - Artigos/Papers |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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mcu223.pdf Acesso restrito! | 2,34 MB | Adobe PDF | Ver/Abrir |