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https://hdl.handle.net/1822/51162
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Campo DC | Valor | Idioma |
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dc.contributor.author | Vilaça, Natália | por |
dc.contributor.author | Totovao, Ricardo | por |
dc.contributor.author | Prasetyanto, Eko Adi | por |
dc.contributor.author | Miranda-Goncalves, Vera | por |
dc.contributor.author | Morais-Santos, Filipa | por |
dc.contributor.author | Fernandes, Rui | por |
dc.contributor.author | Figueiredo, Francisco | por |
dc.contributor.author | Banobre-Lopez, Manuel | por |
dc.contributor.author | Fonseca, A. M. | por |
dc.contributor.author | De Cola, Luisa | por |
dc.contributor.author | Baltazar, Fátima | por |
dc.contributor.author | Neves, Isabel C. | por |
dc.date.accessioned | 2018-02-27T23:10:08Z | - |
dc.date.issued | 2018-01-21 | - |
dc.identifier.citation | Vilaça, Natália; Totovao, Ricardo; Prasetyanto, Eko Adi; Miranda-Goncalves, Vera; Morais-Santos, Filipa; Fernandes, Rui; Figueiredo, Francisco; Banobre-Lopez, Manuel; Fonseca, António M.; De Cola, Luisa; Baltazar, Fatima; Neves, Isabel C., Internalization studies on zeolite nanoparticles using human cells. Journal of Materials Chemistry B, 6, 469-476, 2018 | por |
dc.identifier.issn | 2050-750X | por |
dc.identifier.uri | https://hdl.handle.net/1822/51162 | - |
dc.description.abstract | Zeolites are crystalline porous materials with a regular framework which have non-toxic effects on a variety of human cell lines and have been explored for cell imaging and drug delivery. Understanding the interaction between zeolite nanoparticles and cells is imperative for improving their potentialities, since the process of internalization of these particles is still poorly understood. In this study, the intracellular trafficking and internalization kinetics of zeolite L into breast cancer cells and normal epithelial mammary cells were analysed using scanning electron microscopy (SEM), confocal microscopy and transmission electron microscopy (TEM). We also studied the involvement of endocytic pathways using two pharmacological inhibitors, chlorpromazine and dynasore. Zeolite nanoparticles were taken up by both cell types and the cellular uptake was fast, and started immediately after 5 min of incubation. Interestingly, the uptake was dependent on the cell type since in breast cancer cells it was faster and more efficient, with a higher number of nanoparticles being internalized by cancer cells over time, compared to that in the epithelial mammary cells. TEM results showed that the internalized nanoparticles were mainly localized in the cell vacuoles. The data obtained upon using endocytic pharmacological inhibitors suggest that the zeolite L uptake is mediated by caveolin. | por |
dc.description.sponsorship | N. V. and F. M. S. are recipients of PhD fellowships (SFRH/BD/ 97797/2013, SFRH/BD/87139/2012) from the Foundation for Science and Technology (FCT, Portugal). R. T., E. A. P. and L. D. C. acknowledge the financial support from the European Research Council under the European Union’s Seventh Framework Programme (FP/2007-2013)/ERC Grant Agreement n. [2009-247365]. VM-G is a postdoctoral research fellow from the strategic funding of NORTE-01-0145-FEDER-000027 (ESTIMA-8). This work has been developed under the scope of the project NORTE-01-0145-FEDER-000013 and the project BioTecNorte (operation NORTE-01-0145-FEDER-000004), supported by the Northern Portugal Regional Operational Programme (NORTE 2020), under the Portugal 2020 Partnership Agreement, through the European Regional Development Fund (FEDER). This work has been funded by FEDER funds, through the Competitiveness Factors Operational Programme (COMPETE), and by National funds, through the Foundation for Science and Technology (FCT), under the scope of the projects: PTDC/AAGTEC/5269/ 2014, Centre of Chemistry (UID/QUI/00686/2013 and UID/QUI/ 0686/2016) and ICVS/3B’s POCI-01-0145-FEDER-007038. | por |
dc.language.iso | eng | por |
dc.publisher | Royal Society of Chemistry | por |
dc.relation | info:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBD%2F97797%2F2013/PT | por |
dc.relation | info:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBD%2F87139%2F2012/PT | por |
dc.relation | info:eu-repo/grantAgreement/EC/FP7/247365/EU | por |
dc.relation | PTDC/AAGTEC/5269/2014 | por |
dc.relation | UID/QUI/00686/2013 | por |
dc.relation | UID/QUI/0686/2016 | por |
dc.rights | restrictedAccess | por |
dc.title | Internalization studies on zeolite nanoparticles using human cells | por |
dc.type | article | - |
dc.peerreviewed | yes | por |
dc.relation.publisherversion | http://pubs.rsc.org/en/journals/journal/tb | por |
dc.comments | CEB47372 | por |
oaire.citationStartPage | 469 | por |
oaire.citationEndPage | 476 | por |
oaire.citationIssue | 3 | por |
oaire.citationVolume | 6 | por |
dc.date.updated | 2018-01-20T13:30:04Z | - |
dc.identifier.eissn | 2050-7518 | por |
dc.identifier.doi | 10.1039/C7TB02534C | por |
dc.identifier.pmid | 32254526 | por |
dc.description.publicationversion | info:eu-repo/semantics/publishedVersion | por |
dc.subject.wos | Science & Technology | por |
sdum.journal | Journal of Materials Chemistry B | por |
Aparece nas coleções: | CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series ICVS - Artigos em revistas internacionais / Papers in international journals |
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document_47372_1.pdf Acesso restrito! | 2,9 MB | Adobe PDF | Ver/Abrir |