Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/50155
Título: | RAF-1 promotes survival of thyroid cancer cells harboring RET/PTC1 rearrangement independently of ERK activation |
Autor(es): | Castro, Lisandra Alves, Sara Chaves, Susana R. Costa, Jose Luis Soares, Paula Preto, Ana |
Palavras-chave: | Thyroid cancer RAF-1 BRAF RET/PTC1 PI3K-AKT ERK |
Data: | 5-Nov-2015 |
Editora: | Elsevier 1 |
Revista: | Molecular and Cellular Endocrinology |
Resumo(s): | Thyroid cancer (TC) is frequently associated with BRAF or RAS oncogenic mutations and RET/PTC rear-rangements, with aberrant RAF-MEK-ERK and/or PI3K pathway activation. BRAF underlies ERR activation in most TC cells, but not in TPC-1 cells with RET/PTC1 rearrangement. Here, we show that depletion of RAF-1, a RAF family member with a poorly defined role in TC, decreases proliferation and increases apoptosis in TPC-1 cells and, less significantly, in cells harboring a BRAF(V600E) or HRAS(G13R) mutations, but without affecting ERR activation. We further demonstrate that constitutive activation of ERKs in TPC-1 cells is not caused by mutations in 50 oncogenes and tumor suppressors prone to activate the ERR pathway, or affected by inhibition of BRAF, MEK1/2 or PI3K. Our data indicate that RAF-1 is important for the survival of TPC-1 cells independently of the classical MEK1/2-ERK activation, offering new perspectives on RET/PTC signaling and for the therapy of thyroid cancers. (C) 2015 Elsevier Ireland Ltd. All rights reserved. |
Tipo: | Artigo |
URI: | https://hdl.handle.net/1822/50155 |
DOI: | 10.1016/j.mce.2015.08.006 |
ISSN: | 0303-7207 |
e-ISSN: | 1872-8057 |
Arbitragem científica: | yes |
Acesso: | Acesso restrito autor |
Aparece nas coleções: | DBio - Artigos/Papers |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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Castro L MCE last 1-s2.0-S0303720715300460-main 22 set15.pdf Acesso restrito! | 3,45 MB | Adobe PDF | Ver/Abrir |