Please use this identifier to cite or link to this item: http://hdl.handle.net/1822/49237

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dc.contributor.authorLobo, Jorgepor
dc.contributor.authorFerreira, Maria S.por
dc.contributor.authorAntunes, Ilisa C.por
dc.contributor.authorTeixeira, Marcos André Machado Limapor
dc.contributor.authorBorges, Luisa M. S.por
dc.contributor.authorSousa, Ronaldo Gomespor
dc.contributor.authorGomes, Pedro A.por
dc.contributor.authorCosta, Maria Helenapor
dc.contributor.authorCunha, Marina R.por
dc.contributor.authorCosta, Filipe O.por
dc.date.accessioned2018-01-15T11:21:57Z-
dc.date.issued2017-02-
dc.identifier.issn0831-2796por
dc.identifier.urihttp://hdl.handle.net/1822/49237-
dc.description.abstractIn this study we compared DNA barcode-suggested species boundaries with morphology-based species identifications in the amphipod fauna of the southern European Atlantic coast. DNA sequences of the cytochrome c oxidase subunit I barcode region (COI-5P) were generated for 43 morphospecies (178 specimens) collected along the Portuguese coast which, together with publicly available COI-5P sequences, produced a final dataset comprising 68 morphospecies and 295 sequences. Seventy-five BINs (Barcode Index Numbers) were assigned to these morphospecies, of which 48 were concordant (i.e., 1 BIN = 1 species), 8 were taxonomically discordant, and 19 were singletons. Twelve species had matching sequences (<2% distance) with conspecifics from distant locations (e.g., North Sea). Seven morphospecies were assigned to multiple, and highly divergent, BINs, including specimens of Corophium multisetosum (18% divergence) and Dexamine spiniventris (16% divergence), which originated from sampling locations on the west coast of Portugal (only about 36 and 250 km apart, respectively). We also found deep divergence (4%-22%) among specimens of seven species from Portugal compared to those from the North Sea and Italy. The detection of evolutionarily meaningful divergence among populations of several amphipod species from southern Europe reinforces the need for a comprehensive re-assessment of the diversity of this faunal group.por
dc.description.abstractDans ce travail, les auteurs ont comparé la définition des espèces basée sur les codes a` barres de l’ADN avec celle issue de l’examen morphologique chez les amphipodes de la côte atlantique en Europe méridionale. Les séquences d’ADN de la région-code au sein de la sous-unité I de la cytochrome c oxydase (COI-5P) ont été obtenues pour 43 morpho-espèces (178 spécimens) recueillis le long de la côte du Portugal qui, combinées avec des séquences COI-5P déja` disponibles, ont produit des données représentant 68 morpho-espèces et 295 séquences. Soixante-quinze BIN (« Barcode Index Numbers ») ont été assignés a` ces morpho-espèces, dont 48 étaient concordants (i.e. 1 BIN = 1 espèce), 8 étaient discordants et 19 étaient uniques. Douze espèces avaient des séquences très semblables (distance <2 %) avec des espèces conspécifiques lointaines (p. ex. en Mer du Nord). Sept morpho-espèces ont été assignées a` plusieurs BIN très divergents, incluant des spécimens du Corophium multisetosum (divergence de 18 %) et duDexamine spiniventris(divergence de 16 %), lesquels provenaient de sites d’échantillonnage sur la côte ouest du Portugal situés a` seulement 36 et 250 km l’un de l’autre, respectivement. Les auteurs ont également trouvé une divergence importante (4 a` 22 %) entre spécimens du Portugal et de la Mer du Nord ou de l’Italie au sein de sept espèces. La détection de divergences significatives au plan évolutif entre populations de plusieurs espèces d’amphipodes de l’Europe méridionale confirme la nécessité de réexaminer de manière exhaustive la diversité au sein de ce groupe.por
dc.description.sponsorshipThis work was supported by FEDER through POFCCOMPETE and by national funds from “Fundação para a Ciência e a Tecnologia (FCT)” in the scope of the grants FCOMP-01-0124-FEDER-015429, PEst-OE/BIA/UI4050/2014, PEst-C/MAR/UI0284/2014, PEst-C/MAR/LA0017/2013, and UID/AMB/50017/2013. Sequencing at the Biodiversity Institute of Ontario was supported by funding to the International Barcode of Life Project (iBOL) through the Canadian Centre for DNA Barcoding, from the Ontario Genomics Institute, Genome Canada, the Ontario Ministry of Research and Innovation, and the Natural Sciences and Engineering Research Council of Canada. Jorge Lobo was supported by a PhD fellowship (SFRH/BD/69750/2010) from FCT.por
dc.language.isoengpor
dc.publisherNRC Research Press (Canadian Science Publishing)por
dc.relationinfo:eu-repo/grantAgreement/FCT/5876/135919/PTpor
dc.relationinfo:eu-repo/grantAgreement/FCT/COMPETE/132951/PTpor
dc.relationinfo:eu-repo/grantAgreement/FCT/5876/147273/PTpor
dc.relationinfo:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBD%2F69750%2F2010/PTpor
dc.rightsrestrictedAccesspor
dc.subjectAmphipodapor
dc.subjectAnimalspor
dc.subjectDNA, Mitochondrialpor
dc.subjectElectron Transport Complex IVpor
dc.subjectEuropepor
dc.subjectEvolution, Molecularpor
dc.subjectGenetic Variationpor
dc.subjectGenetics, Populationpor
dc.subjectGeographypor
dc.subjectPhylogenypor
dc.subjectDNA Barcoding, Taxonomicpor
dc.subjectCryptic speciespor
dc.subjectCytochrome c oxidase subunit Ipor
dc.subjectDNA barcode reference librarypor
dc.subjectEspèces cryptiquespor
dc.subjectSous-unité I de la cytochrome c oxydasepor
dc.subjectBanque de données de référence de codes à barres de l’ADNpor
dc.titleContrasting morphological and DNA barcode-suggested species boundaries among shallow-water amphipod fauna from the southern European Atlantic coastpor
dc.typearticlepor
dc.peerreviewedyespor
oaire.citationStartPage147por
oaire.citationEndPage157por
oaire.citationIssue2por
oaire.citationVolume60por
dc.identifier.essn1480-3321por
dc.identifier.doi10.1139/gen-2016-0009por
dc.identifier.pmid28044453por
dc.description.publicationversioninfo:eu-repo/semantics/publishedVersionpor
dc.subject.wosScience & Technologypor
sdum.journalGenomepor
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