Please use this identifier to cite or link to this item: http://hdl.handle.net/1822/48148

TitleCrosstalk between adipose stem cells and tendon cells reveals a temporal regulation of tenogenesis by matrix deposition and remodeling
Author(s)Almeida, Raquel Costa
Calejo, Isabel
Reis, R. L.
Gomes, Manuela E.
KeywordsAdipose-derived stem cells
Co-culture
Extracellular matrix
Matrix metalloproteinases
Temporal gene regulation
Tendon Regeneration
Tenogenesis
Issue date2018
PublisherWiley
JournalJournal of Cellular Physiology
CitationCosta-Almeida R., Calejo I., Reis R. L., Gomes M. E. Crosstalk between adipose stem cells and tendon cells reveals a temporal regulation of tenogenesis by matrix deposition and remodeling, Journal Of Cellular Physiology, doi:10.1002/jcp.26363, 2017
Abstract(s)Tendon injuries constitute an unmet clinical challenge owing to the limited intrinsic regenerative ability of this tissue. Cell-based therapies aim at improving tendon healing through the delicate orchestration of tissue rebuilding and regain of function. Hence, human adipose-derived stem cells (hASCs) have been proposed as a promising cell source for boosting tendon regeneration. In this work, we investigated the influence of hASCs on native human tendon-derived cells (hTDCs) through the establishment of a direct contact co-culture system. Results demonstrated that direct interactions between these cell types resulted in controlled proliferation and spontaneous cell elongation. ECM-related genes, particularly COL1A1 and TNC, and genes involved in ECM remodeling, such as MMP1, MMP2, MMP3 and TIMP1, were expressed in co-cultures in a temporally regulated manner. In addition, deposition of collagen type I was accelerated in co-cultures systems and favored over the production of collagen type III, resulting in an enhanced COL1/COL3 ratio as soon as 7 days. In conclusion, hASCs seem to be good candidates in modulating the behavior of native tendon cells, particularly through a balanced process of ECM synthesis and degradation.
TypeArticle
URIhttp://hdl.handle.net/1822/48148
DOI10.1002/jcp.26363
ISSN0021-9541
e-ISSN1097-4652
Publisher versionhttp://onlinelibrary.wiley.com/doi/10.1002/jcp.26363/full
Peer-Reviewedyes
AccessRestricted access (UMinho)
Appears in Collections:3B’s - Artigos em revistas/Papers in scientific journals

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