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https://hdl.handle.net/1822/46854
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Campo DC | Valor | Idioma |
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dc.contributor.author | Reys, L. L. | por |
dc.contributor.author | Silva, Simone Santos | por |
dc.contributor.author | Pirraco, Rogério P. | por |
dc.contributor.author | Marques, A. P. | por |
dc.contributor.author | Mano, J. F. | por |
dc.contributor.author | Silva, Tiago H. | por |
dc.contributor.author | Reis, R. L. | por |
dc.date.accessioned | 2017-10-26T14:44:36Z | - |
dc.date.available | 2019-09-01T06:00:53Z | - |
dc.date.issued | 2017-08 | - |
dc.date.submitted | 2017-08 | - |
dc.identifier.citation | Reys L. L., Silva S. S., Pirraco R. P., Marques A. P., Mano J. F., Silva T. H., Reis R. L. Influence of freezing temperature and deacetylation degree on the performance of freeze-dried chitosan scaffolds towards cartilage tissue engineering, European Polymer Journal, Vol. 95, Issue C, pp. 232-240, doi:10.1016/j.eurpolymj.2017.08.017, 2017 | por |
dc.identifier.issn | 0014-3057 | por |
dc.identifier.uri | https://hdl.handle.net/1822/46854 | - |
dc.description.abstract | Chitosan-based porous structures have been significantly studied across the world as potential tissue engineering scaffolds. Despite the differences in chitosan produced from squid pens or crustacean shells, with the former being more reactive and easily available with a higher degree of deacetylation (DD), most of the studies report the use of crab or shrimp chitosan as they are readily available commercial sources. The aim of this work was to highlight the great potential of chitosan produced from squid pens for biomedical application. From freeze-dried scaffolds for soft tissue engineering, we investigated the influence of the DD of chitosan and the freezing temperature during processing on their performance. Chitosan was obtained by deacetylation of β-chitin previously isolated from endoskeleton of giant squid Dosidicus gigas (DD 91.2%) and compared with a commercially available batch obtained from crab shells (DD 76.6%). Chitosan solutions were frozen at â 80° C or â 196° C and further freeze-dried to obtain 3D porous structures (scaffolds). Scaffolds prepared at â 196° C have a compact structure with smaller pores, while those prepared at â 80° C showed a lamellar structure with larger pores. The compressive modulus varied from 0.7 up to 8.8 MPa. Both types of scaffolds were stable on PBS, including in the presence of lysozyme, up to 4 weeks. Furthermore, the squid chitosan scaffolds processed at â 80° C promoted ATDC5 chondrocyte-like cells adhesion and proliferation. The results suggest that the developed squid chitosan scaffolds might be further exploited for ap- plications in cartilage tissue engineering. | por |
dc.description.sponsorship | This work was partially funded by ERDF through POCTEP Projects 0330_IBEROMARE_1_P and 0687_NOVOMAR_1_P, Atlantic Area Project 2011-1/164 MARMED and by European Union through European Research Council – Project ComplexiTE (ERC-2012-ADG 20120216-321266). Portuguese Foundation for Science and Technology is gratefully acknowledged for post-doc grants of R.P. Pirraco (SFRH/BPD/101886/2014) and S.S. Silva (SFRH/BPD/112140/2015) and PhD grant of Lara L. Reys (SFRH/BD/112139/2015). The authors would also like to acknowledge to Dr. Julio Maroto, from Fundación CETMAR (Spain) and Roi Vilela, from PESCANOVA S.A. (Spain), for the kind offer of squid pens. | por |
dc.language.iso | eng | por |
dc.publisher | Elsevier 1 | por |
dc.relation | 0330_IBEROMARE_1_P | por |
dc.relation | 0687_NOVOMAR_1_P | por |
dc.relation | 2011-1/164 | por |
dc.relation | ERC-2012-ADG 20120216-321266 | por |
dc.relation | SFRH/BPD/101886/2014 | por |
dc.relation | SFRH/BPD/112140/2015 | por |
dc.relation | SFRH/BD/112139/2015 | por |
dc.rights | openAccess | por |
dc.subject | Degree of deacetylation | por |
dc.subject | Freeze-drying | por |
dc.subject | Freezing temperature and Cartilage | por |
dc.subject | Marine biomaterials | por |
dc.subject | Scaffolds | por |
dc.subject | Squid chitosan | por |
dc.subject | Tissue engineering | por |
dc.subject | β-chitin | por |
dc.subject | beta-chitin | por |
dc.subject | Freezing temperature | por |
dc.subject | Cartilage | por |
dc.title | Influence of freezing temperature and deacetylation degree on the performance of freeze-dried chitosan scaffolds towards cartilage tissue engineering | por |
dc.type | article | - |
dc.peerreviewed | yes | por |
dc.relation.publisherversion | http://www.sciencedirect.com/science/article/pii/S0014305717307462 | por |
dc.comments | http://3bs.uminho.pt/node/19171 | por |
oaire.citationStartPage | 232 | por |
oaire.citationEndPage | 240 | por |
oaire.citationIssue | C | por |
oaire.citationVolume | 95 | por |
dc.date.updated | 2017-09-25T14:37:28Z | - |
dc.identifier.doi | 10.1016/j.eurpolymj.2017.08.017 | por |
dc.description.publicationversion | info:eu-repo/semantics/publishedVersion | por |
dc.subject.wos | Science & Technology | por |
sdum.journal | European Polymer Journal | por |
Aparece nas coleções: | 3B’s - Artigos em revistas/Papers in scientific journals |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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19171-Influence of freezing temperature and deacetylation degree on the performance of freeze-dried chitosan scaffolds towards cartilage tissue engineering.pdf | 1,11 MB | Adobe PDF | Ver/Abrir |