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dc.contributor.authorReys, L. L.por
dc.contributor.authorSilva, Simone Santospor
dc.contributor.authorPirraco, Rogério P.por
dc.contributor.authorMarques, A. P.por
dc.contributor.authorMano, J. F.por
dc.contributor.authorSilva, Tiago H.por
dc.contributor.authorReis, R. L.por
dc.date.accessioned2017-10-26T14:44:36Z-
dc.date.issued2017-08-
dc.date.submitted2017-08-
dc.identifier.citationReys L. L., Silva S. S., Pirraco R. P., Marques A. P., Mano J. F., Silva T. H., Reis R. L. Influence of freezing temperature and deacetylation degree on the performance of freeze-dried chitosan scaffolds towards cartilage tissue engineering, European Polymer Journal, Vol. 95, Issue C, pp. 232-240, doi:10.1016/j.eurpolymj.2017.08.017, 2017por
dc.identifier.issn0014-3057por
dc.identifier.urihttp://hdl.handle.net/1822/46854-
dc.description.abstractChitosan-based porous structures have been significantly studied across the world as potential tissue engineering scaffolds. Despite the differences in chitosan produced from squid pens or crustacean shells, with the former being more reactive and easily available with a higher degree of deacetylation (DD), most of the studies report the use of crab or shrimp chitosan as they are readily available commercial sources. The aim of this work was to highlight the great potential of chitosan produced from squid pens for biomedical application. From freeze-dried scaffolds for soft tissue engineering, we investigated the influence of the DD of chitosan and the freezing temperature during processing on their performance. Chitosan was obtained by deacetylation of β-chitin previously isolated from endoskeleton of giant squid Dosidicus gigas (DD 91.2%) and compared with a commercially available batch obtained from crab shells (DD 76.6%). Chitosan solutions were frozen at â 80° C or â 196° C and further freeze-dried to obtain 3D porous structures (scaffolds). Scaffolds prepared at â 196° C have a compact structure with smaller pores, while those prepared at â 80° C showed a lamellar structure with larger pores. The compressive modulus varied from 0.7 up to 8.8 MPa. Both types of scaffolds were stable on PBS, including in the presence of lysozyme, up to 4 weeks. Furthermore, the squid chitosan scaffolds processed at â 80° C promoted ATDC5 chondrocyte-like cells adhesion and proliferation. The results suggest that the developed squid chitosan scaffolds might be further exploited for ap- plications in cartilage tissue engineering.por
dc.description.sponsorshipThis work was partially funded by ERDF through POCTEP Projects 0330_IBEROMARE_1_P and 0687_NOVOMAR_1_P, Atlantic Area Project 2011-1/164 MARMED and by European Union through European Research Council – Project ComplexiTE (ERC-2012-ADG 20120216-321266). Portuguese Foundation for Science and Technology is gratefully acknowledged for post-doc grants of R.P. Pirraco (SFRH/BPD/101886/2014) and S.S. Silva (SFRH/BPD/112140/2015) and PhD grant of Lara L. Reys (SFRH/BD/112139/2015). The authors would also like to acknowledge to Dr. Julio Maroto, from Fundación CETMAR (Spain) and Roi Vilela, from PESCANOVA S.A. (Spain), for the kind offer of squid pens.por
dc.language.isoengpor
dc.publisherElsevierpor
dc.relation0330_IBEROMARE_1_Ppor
dc.relation0687_NOVOMAR_1_Ppor
dc.relation2011-1/164por
dc.relationERC-2012-ADG 20120216-321266por
dc.relationSFRH/BPD/101886/2014por
dc.relationSFRH/BPD/112140/2015por
dc.relationSFRH/BD/112139/2015por
dc.rightsembargoedAccess (2 Years)por
dc.subjectDegree of deacetylationpor
dc.subjectFreeze-dryingpor
dc.subjectFreezing temperature and Cartilagepor
dc.subjectMarine biomaterialspor
dc.subjectScaffoldspor
dc.subjectSquid chitosanpor
dc.subjectTissue engineeringpor
dc.subjectβ-chitinpor
dc.subjectbeta-chitinpor
dc.subjectFreezing temperaturepor
dc.subjectCartilagepor
dc.titleInfluence of freezing temperature and deacetylation degree on the performance of freeze-dried chitosan scaffolds towards cartilage tissue engineeringpor
dc.typearticle-
dc.peerreviewedyespor
dc.relation.publisherversionhttp://www.sciencedirect.com/science/article/pii/S0014305717307462por
dc.commentshttp://3bs.uminho.pt/node/19171por
degois.publication.firstPage232por
degois.publication.lastPage240por
degois.publication.issueCpor
degois.publication.volume95por
dc.date.updated2017-09-25T14:37:28Z-
dc.identifier.doi10.1016/j.eurpolymj.2017.08.017por
dc.date.embargo2019-09-01-
dc.description.publicationversioninfo:eu-repo/semantics/publishedVersionpor
dc.subject.wosScience & Technologypor
sdum.journalEuropean Polymer Journalpor
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