Utilize este identificador para referenciar este registo: http://hdl.handle.net/1822/46851

TítuloUncovering the effect of low-frequency static magnetic field on tendon-derived cells: from mechanosensing to tenogenesis
Autor(es)Pesqueira, T.
Almeida, Raquel Costa
Gomes, Manuela E.
Palavras-chaveContact-free therapy
Immunomodulation
Magnetotherapy
Remote actuation
Tendon mechanotransduction
Tendon-related markers
DataSet-2017
EditoraNature Publishing Group
RevistaScientific Reports
CitaçãoPesqueira T., Costa-Almeida R., Gomes M. E. Uncovering the effect of low-frequency static magnetic field on tendon-derived cells: from mechanosensing to tenogenesis, Scientific Reports, Vol. 7, pp. 10948, doi:10.1038/s41598-017-11253-6, 2017
Resumo(s)Magnetotherapy has been receiving increased attention as an attractive strategy for modulating cell physiology directly at the site of injury, thereby providing the medical community with a safe and non- invasive therapy. Yet, how magnetic eld in uences tendon cells both at the cellular and molecular levels remains unclear. Thus, the in uence of a low-frequency static magnetic eld (2 Hz, 350 mT) on human tendon-derived cells was studied using di erent exposure times (4 and 8 h; short-term studies) and di erent regimens of exposure to an 8h-period of magnetic stimulation (continuous, every 24 h or every 48 h; long-term studies). Herein, 8 h stimulation in short-term studies signi cantly upregulated the expression of tendon-associated genes SCX, COL1A1, TNC and DCN (p < 0.05) and altered intracellular Ca2+ levels (p < 0.05). Additionally, every 24 h regimen of stimulation signi cantly upregulated COL1A1, COL3A1 and TNC at day 14 in comparison to control (p < 0.05), whereas continuous exposure di erentially regulated the release of the immunomodulatory cytokines IL-1β and IL-10 (p < 0.001) but only at day 7 in comparison to controls. Altogether, these results provide new insights on how low-frequency static magnetic eld ne-tune the behaviour of tendon cells according to the magnetic settings used, which we foresee to represent an interesting candidate to guide tendon regeneration.
Tipoarticle
URIhttp://hdl.handle.net/1822/46851
DOI10.1038/s41598-017-11253-6
ISSN2045-2322
e-ISSN2045-2322
Versão da editorahttps://www.nature.com/articles/s41598-017-11253-6
Arbitragem científicayes
AcessoopenAccess
Aparece nas coleções:3B’s - Artigos em revistas/Papers in scientific journals

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