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https://hdl.handle.net/1822/44514
Título: | Genome analysis and physiological comparison of Alicycliphilus denitrificans strains BC and K601T |
Autor(es): | Oosterkamp, Margreet J. Veuskens, Teun Talarico Saia, Flávia Weelink, Sander A. B. Goodwin, Lynne A. Daligault, Hajnalka E. Bruce, David C. Detter, John C. Tapia, Roxanne Han, Cliff S. Land, Miriam L. Hauser, Loren J. Langenhoff, Alette A. M. Gerritse, Jan van Berkel, Willem J. H. Pieper, Dietmar H. Junca, Howard Smidt, Hauke Schraa, Gosse Davids, Mark Schaap, Peter J. Plugge, Caroline M. Stams, Alfons Johannes Maria |
Data: | Jun-2013 |
Editora: | Public Library of Science |
Revista: | PLoS ONE |
Citação: | Oosterkamp, Margreet J.; Veuskens, Teun; Talarico Saia, Flávia; Weelink, Sander A. B.; Goodwin, Lynne A.; Daligault, Hajnalka E.; Bruce, David C.; Detter, John C.; Tapia, Roxanne; Han, Cliff S.; Land, Miriam L.; Hauser, Loren J.; Langenhoff, Alette A. M.; Gerritse, Jan; van Berkel, Willem J. H.; Pieper, Dietmar H.; Junca, Howard; Smidt, Hauke; Schraa, Gosse; Davids, Mark; Schaap, Peter J.; Plugge, Caroline M.; Stams, A. J. M., Genome analysis and physiological comparison of Alicycliphilus denitrificans strains BC and K601T. PLoS One, 8(6), e66971, 2013 |
Resumo(s): | The genomes of the Betaproteobacteria Alicycliphilus denitrificans strains BC and K601T have been sequenced to get insight into the physiology of the two strains. Strain BC degrades benzene with chlorate as electron acceptor. The cyclohexanol-degrading denitrifying strain K601T is not able to use chlorate as electron acceptor, while strain BC cannot degrade cyclohexanol. The 16S rRNA sequences of strains BC and K601T are identical and the fatty acid methyl ester patterns of the strains are similar. Basic Local Alignment Search Tool (BLAST) analysis of predicted open reading frames of both strains showed most hits with Acidovorax sp. JS42, a bacterium that degrades nitro-aromatics. The genomes include strain-specific plasmids (pAlide201 in strain K601T and pAlide01 and pAlide02 in strain BC). Key genes of chlorate reduction in strain BC were located on a 120 kb megaplasmid (pAlide01), which was absent in strain K601T. Genes involved in cyclohexanol degradation were only found in strain K601T. Benzene and toluene are degraded via oxygenase-mediated pathways in both strains. Genes involved in the meta-cleavage pathway of catechol are present in the genomes of both strains. Strain BC also contains all genes of the ortho-cleavage pathway. The large number of mono- and dioxygenase genes in the genomes suggests that the two strains have a broader substrate range than known thus far. |
Tipo: | Artigo |
URI: | https://hdl.handle.net/1822/44514 |
DOI: | 10.1371/journal.pone.0066971 |
ISSN: | 1932-6203 |
e-ISSN: | 1932-6203 |
Versão da editora: | http://journals.plos.org/plosone/ |
Arbitragem científica: | yes |
Acesso: | Acesso aberto |
Aparece nas coleções: | CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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document_46642_1.pdf | 2,15 MB | Adobe PDF | Ver/Abrir |