Please use this identifier to cite or link to this item: http://hdl.handle.net/1822/4401

TitleOptimisation of a serine protease coupling to Eudragit S-100 by experimental design techniques
Author(s)Silva, Carla J. S. M.
Gübitz, Georg M.
Paulo, Artur Cavaco
KeywordsSerine proteases
Eudragit
Coupling
Factorial designs
Issue date2006
PublisherSociety of Chemical Industry
JournalJournal of Chemical Technology and Biotechnology
CitationJournal of Chemical Technology and Biotechnology." ISSN 0268–2575. 81:8 (2006) 8-16.
Abstract(s)A full factorial design was used to study the influence of four different variables, namely polymer concentration, carbodiimide concentration, time of reaction and blocking agent concentration, on the coupling of a serine protease into a soluble–insoluble polymer (Eudragit S-100). All of the four factors studied have played a critical role in the protease coupling. Response surface methodology was used as an optimisation strategy to attain a conjugate with high activity yield and operational stability at 60 ◦C. Under optimised conditions (Eudragit, 2.5% w/v, carbodiimide, 0.2% w/v, coupling time, 1 h and blocking agent concentration, 0.05%), the conjugate activity yield was about 45% and its operational stability at 60 ◦C was increased by 1.7 times. After reusing the conjugate for five cycles, the remaining activity was still 72% of the initial value when compared with the native enzyme. Several tests confirmed that the enzyme was covalently crosslinked to Eudragit, which represents an improvement in the carbodiimide coupling of proteases into soluble–insoluble polymers.
TypeArticle
URIhttp://hdl.handle.net/1822/4401
DOI10.1002/jctb.1350
ISSN0268–2575
Peer-Reviewedyes
AccessOpen access
Appears in Collections:DET/2C2T - Artigos em revistas internacionais com arbitragem científica

Files in This Item:
File Description SizeFormat 
78.pdf185,23 kBAdobe PDFView/Open

Partilhe no FacebookPartilhe no TwitterPartilhe no DeliciousPartilhe no LinkedInPartilhe no DiggAdicionar ao Google BookmarksPartilhe no MySpacePartilhe no Orkut
Exporte no formato BibTex mendeley Exporte no formato Endnote Adicione ao seu ORCID