Utilize este identificador para referenciar este registo: http://hdl.handle.net/1822/43913

TítuloGrowth factor-free pre-vascularization of cell sheets for tissue engineering
Autor(es)Costa, M.
Pirraco, Rogério P.
Cerqueira, Mariana Teixeira
Reis, R. L.
Marques, A. P.
Palavras-chaveAdipose tissue
Cell sheets
Growth factor-free
Hypoxia
Stromal vascular fraction
Vascularization
DataJun-2016
EditoraSpringer
CitaçãoCosta M., Pirraco R. P., Cerqueira M. T., Reis R. L., Marques A. P. Growth Factor-Free Pre-vascularization of Cell Sheets for Tissue Engineering., Stem Cell Heterogeneity: Methods and Protocols, Vol. 1516, pp. 219 - 226, doi:10.1007/7651_2016_362, 2016
Resumo(s)The therapeutic efficacy of tissue-engineered constructs is often compromised by inadequate inosculation and neo-vascularization. This problem is considered one of the biggest hurdles in the field and finding a solution is currently the focus of a great fraction of the research community. Many of the methodologies designed to address this issue propose the use of endothelial cells and angiogenic growth factors, or combinations of both, to accelerate neo-vascularization after transplantation. However, an adequate solution is still elusive. In this context, we describe a methodology that combines the use of the stromal vascular fraction (SVF) isolated from adipose tissue with low oxygen culture to produce pre-vascularized cell sheets as angiogenic tools for Tissue Engineering. The herein proposed approach takes advantage of the SVF angiogenic nature conferred by adipose stem cells, endothelial progenitors, endothelial and hematopoietic cells, and pericytes and further potentiates it using low oxygen, or hypoxic, culture. Freshly isolated nucleated SVF cells are cultured in hyperconfluent conditions under hypoxia (pO2â =â 5â %) for up to 5 days in medium without extrinsic growth factors enabling the generation of contiguous sheets as described by the cell sheet engineering technique. Flow cytometry and immunocytochemistry allow confirming the phenotype of the different cell types composing the cell-sheets as well the organization of the CD31(+) cells in branched and highly complex tube-like structures. Overall, a simple and flexible approach to promote growth factor-free pre-vascularization of cell sheets for tissue engineering (TE) applications is described.
TipobookPart
URIhttp://hdl.handle.net/1822/43913
ISBN978-1-4939-6550-2
DOI10.1007/7651_2016_362
ISSN1064-3745
Versão da editorahttp://link.springer.com/protocol/10.1007%2F7651_2016_362
Arbitragem científicayes
AcessorestrictedAccess
Aparece nas coleções:3B’s - Capítulos de Livros/Book Chapters

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