Please use this identifier to cite or link to this item: http://hdl.handle.net/1822/43825

TitleExtracellular vesicles derived from osteogenically induced human bone marrow mesenchymal stem cells can modulate lineage commitment
Author(s)Martins, Margarida Isabel Barros Coelho
Ribeiro, Diana Margarida da Costa
Martins, Albino
Reis, R. L.
Neves, N. M.
KeywordsAdvanced therapies/ bone regeneration
Differentiation
Exosome
Osteogenic commitment strategies
Secretome
Stem cells
Issue dateMar-2016
PublisherCell Press
JournalStem Cell Reports
CitationMartins M., Ribeiro D., Martins A., Reis R. L., Neves N. M. Extracellular Vesicles Derived from Osteogenically Induced Human Bone Marrow Mesenchymal Stem Cells Can Modulate Lineage Commitment, Stem Cell Reports , Vol. 6, Issue 3, pp. 284-291, doi:10.1016/j.stemcr.2016.01.001, 2016
Abstract(s)The effective osteogenic commitment of human bone marrow mesenchymal stem cells (hBMSCs) is critical for bone regenerative therapies. Extracellular vesicles (EVs) derived from hBMSCs have a regenerative potential that has been increasingly recognized. Herein, the osteoinductive potential of osteogenically induced hBMSC-EVs was examined. hBMSCs secreted negatively charged nanosized vesicles (⠼35 nm) with EV-related surface markers. The yield of EVs over 7 days was dependent on an osteogenic stimulus (standard chemical cocktail or RUNX2 cationic-lipid transfection). These EVs were used to sequentially stimulate homotypic uncommitted cells during 7 days, matching the seeding density of EV parent cells, culture time, and stimuli. Osteogenically committed hBMSC-EVs induced an osteogenic phenotype characterized by marked early induction of BMP2, SP7, SPP1, BGLAP/IBSP, and alkaline phosphatase. Both EV groups outperformed the currently used osteoinductive strategies. These data show that naturally secreted EVs can guide the osteogenic commitment of hBMSCs in the absence of other chemical or genetic osteoinductors.
TypeArticle
URIhttp://hdl.handle.net/1822/43825
DOI10.1016/j.stemcr.2016.01.001
ISSN2213-6711
Publisher versionhttp://www.cell.com/stem-cell-reports/pdf/S2213-6711(16)00002-3.pdf
Peer-Reviewedyes
AccessOpen access
Appears in Collections:3B’s - Artigos em revistas/Papers in scientific journals

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