Utilize este identificador para referenciar este registo: http://hdl.handle.net/1822/43699

TítuloA radially organized multipatterned device as a diagnostic tool for the screening of topographies in tissue engineering biomaterials
AutorBabo, Pedro Miguel Sousa
Klymov, Alexey
teRiet, Joost
Reis, R. L.
Jansen, John A.
Gomes, Manuela E.
Walboomers, X. Frank
Palavras-chaveAnisotropic alignment
Nanogrooves
Platelet lysate
DataSet-2016
EditoraMary Ann Liebert
CitaçãoBabo P. S., Klymov A., teRiet J., Reis R. L., Jansen J. A., Gomes M. E., Walboomers X. F. A radially organized multi-patterned device as a diagnostic tool for the screening of topographies in tissue engineering biomaterials, Tissue Engineering: part C, doi:10.1089/ten.tec.2016.0224, 2016
ResumoMicro- and nanotextured biomaterial surfaces have been widely studied for their capacity to drive the regeneration of organized tissues. Nanotopographical features in the shape of grooveâ ridge patterns aim at mimicking the extracellular matrix organization. However, to date, a wide array of groove and ridge sizes has been described. In this work, we therefore tested a device composed of a multipatterned array consisting of six patterns of radially arranged parallel nanogrooves, with a pitch ranging from 0 to 1000nm and a depth ranging from 0 to 170 nm, to be used as a tool for the expeditious and simultaneous screening of surface topographies aiming the regeneration of anisotropically organized tissues such as ligament. The topographies were reproduced in (1) epoxy resin or (2) membranes produced by the crosslinking of platelet lysate (PL) with genipin (gPL). Both materials were seeded with periodontal ligament cells (PDLCs) and the proliferation, migration, as well as cell alignment were assessed. The effect of topography in PDLCs was only evident in terms of cell organization, resulting in a highly anisotropic organization of the cells for the 1000 and 600nm patterns, and in an increased isotropic organization for shallower topographies. Overall, our results suggest that this multipatterned system can be a valuable diagnostic tool for biomaterials aiming at the regeneration of anisotropically organized tissues, such as periodontal ligament.
Tipoarticle
URIhttp://hdl.handle.net/1822/43699
DOI10.1089/ten.tec.2016.0224
ISSN2152-4955
Arbitragem científicayes
AcessorestrictedAccess
Aparece nas coleções:3B’s - Artigos em revistas/Papers in scientific journals

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