Utilize este identificador para referenciar este registo: http://hdl.handle.net/1822/43699

TítuloA radially organized multipatterned device as a diagnostic tool for the screening of topographies in tissue engineering biomaterials
Autor(es)Babo, Pedro Miguel Sousa
Klymov, Alexey
teRiet, Joost
Reis, R. L.
Jansen, John A.
Gomes, Manuela E.
Walboomers, X. Frank
Palavras-chaveAnisotropic alignment
Platelet lysate
EditoraMary Ann Liebert
RevistaTissue Engineering: Part C: Methods
CitaçãoBabo P. S., Klymov A., teRiet J., Reis R. L., Jansen J. A., Gomes M. E., Walboomers X. F. A radially organized multi-patterned device as a diagnostic tool for the screening of topographies in tissue engineering biomaterials, Tissue Engineering: part C, doi:10.1089/ten.tec.2016.0224, 2016
Resumo(s)Micro- and nanotextured biomaterial surfaces have been widely studied for their capacity to drive the regeneration of organized tissues. Nanotopographical features in the shape of grooveâ ridge patterns aim at mimicking the extracellular matrix organization. However, to date, a wide array of groove and ridge sizes has been described. In this work, we therefore tested a device composed of a multipatterned array consisting of six patterns of radially arranged parallel nanogrooves, with a pitch ranging from 0 to 1000nm and a depth ranging from 0 to 170 nm, to be used as a tool for the expeditious and simultaneous screening of surface topographies aiming the regeneration of anisotropically organized tissues such as ligament. The topographies were reproduced in (1) epoxy resin or (2) membranes produced by the crosslinking of platelet lysate (PL) with genipin (gPL). Both materials were seeded with periodontal ligament cells (PDLCs) and the proliferation, migration, as well as cell alignment were assessed. The effect of topography in PDLCs was only evident in terms of cell organization, resulting in a highly anisotropic organization of the cells for the 1000 and 600nm patterns, and in an increased isotropic organization for shallower topographies. Overall, our results suggest that this multipatterned system can be a valuable diagnostic tool for biomaterials aiming at the regeneration of anisotropically organized tissues, such as periodontal ligament.
Arbitragem científicayes
Aparece nas coleções:3B’s - Artigos em revistas/Papers in scientific journals

Ficheiros deste registo:
Ficheiro Descrição TamanhoFormato 
18878-ten.tec_.2016.0224-1.pdf789,23 kBAdobe PDFVer/Abrir  Solicitar cópia ao autor!

Partilhe no FacebookPartilhe no TwitterPartilhe no DeliciousPartilhe no LinkedInPartilhe no DiggAdicionar ao Google BookmarksPartilhe no MySpacePartilhe no Orkut
Exporte no formato BibTex mendeley Exporte no formato Endnote Adicione ao seu Currículo DeGóis