Utilize este identificador para referenciar este registo: http://hdl.handle.net/1822/43587

TítuloDual release of a hydrophilic and a hydrophobic osteogenic factor from a single liposome
Autor(es)Monteiro, N.
Martins, A.
Pires, R. A.
Faria, S.
Fonseca, N. A.
Moreira, J. N.
Reis, R. L.
Neves, N. M.
Palavras-chaveHydrophilic
Hydrophobic osteogenic factor
Liposome
DataNov-2016
EditoraThe Royal Society of Chemistry
RevistaRSC Advances
CitaçãoMonteiro N., Martins A., Pires R. A., Faria S., Fonseca N. A., Moreira J. N., Reis R. L., Neves N. M. Dual release of a hydrophilic and a hydrophobic osteogenic factor from a single liposome, Rsc Advances, Vol. 6, Issue 115, pp. 114599-114612, doi:10.1039/C6RA21623D, 2016
Resumo(s)Delivery systems may be designed to protect and control the release kinetics of growth/differentiation factors in a spatiotemporal manner. Liposomes are examples of biological-based bioactive agent delivery systems. In this work, ascorbic acid (AscA) was encapsulated in the inner compartment of the liposome and dexamethasone (Dex) was encapsulated within the lipid bilayer in order to develop a dual release system of these bioactive agents involved in the osteogenic differentiation of mesenchymal stem cells (MSCs). The particle size ($150 nm) of the prepared liposomes showed a monodisperse distribution. The bioactive agent release study showed that Dex was released more rapidly from the liposomes than AscA. The Dex release profile showed an initial burst release within 12 h; afterwards, a slower and sustained release was observed until 21 days. The release of AscA from the liposomes was not detected until 6 h; afterwards, a linear release was observed from 24 h until 21 days. The effect of Dexâ AscA-loaded liposomes on the viability, proliferation and osteogenic differentiation of human bone marrow-derived MSCs (hBMSCs) were assessed. The cell culture results showed that the Dexâ AscA-loaded liposomes (in a single dose or in repeated doses) do not have any cytotoxic effect. Dexâ AscA-loaded liposomes given once did not promote induction of hBMSCs differentiation into the osteogenic lineage. However, Dexâ AscA-loaded liposomes given repeatedly promoted the hBMSCs differentiation into the osteogenic lineage, both in basal medium and complete osteogenic medium. These results were genotypically demonstrated by the expression of osteoblastic markers. In conclusion, Dexâ AscA-loaded liposomes represent a biological nanoparticle strategy with potential safety and efficacy for bone tissue engineering approaches.
Tipoarticle
URIhttp://hdl.handle.net/1822/43587
DOI10.1039/C6RA21623D
ISSN2046-2069
Versão da editorahttp://dx.doi.org/10.1039/C6RA21623D
Arbitragem científicayes
AcessorestrictedAccess
Aparece nas coleções:3B’s - Artigos em revistas/Papers in scientific journals

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