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TitleStaphylococcus epidermidis biofilm-released cells induce a prompt and more marked in vivo inflammatory-type response than planktonic or biofilm cells
Author(s)França, Ângela Maria Oliveira Sousa
Pérez-Cabezas, Begoña
Correia, Alexandra
Pier, G. B.
Cerca, Nuno
Vilanova, Manuel
KeywordsS. epidermidis
biofilm-released cells
splenocytes transcriptome
pro-inflammatory cytokines
tissue colonization
Issue date12-Sep-2016
PublisherFrontiers Media
JournalFrontiers in Microbiology
CitationFrança, Angela; Pérez-Cabezas, Begoña; Correia, Alexandra; Pier, G. B.; Cerca, Nuno; Vilanova, Manuel, Staphylococcus epidermidis biofilm-released cells induce a prompt and more marked in vivo inflammatory-type response than planktonic or biofilm cells. Frontiers in Microbiology, 7(1530), 2016
Abstract(s)Staphylococcus epidermidis biofilm formation on indwelling medical devices is frequently associated with the development of chronic infections. Nevertheless, it has been suggested that cells released from these biofilms may induce severe acute infections with bacteraemia as one of its major associated clinical manifestations. However, how biofilm-released cells interact with the host remains unclear. Here, using a murine model of hematogenously disseminated infection, we characterized the interaction of cells released from S. epidermidis biofilms with the immune system. Gene expression analysis of mouse splenocytes suggested that biofilm-released cells might be particularly effective at activating inflammatory and antigen presenting cells and inducing cellular apoptosis. Furthermore, biofilm-released cells induced a higher production of pro-inflammatory cytokines, in contrast to mice infected with planktonic cells, even though these had a similar bacterial load in livers and spleens. Overall, these results not only provide insights into the understanding of the role of biofilm-released cells in S. epidermidis biofilm-related infections and pathogenesis, but may also help explain the relapsing character of these infections.
Publisher version
AccessOpen access
Appears in Collections:CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series

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