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TitleCombinatorial approaches with selected phytochemicals to increase antibiotic efficacy against Staphylococcus aureus biofilms
Author(s)Abreu, Ana Cristina
Saavedra, Maria José
Simões, Lúcia C.
Simões, Manuel
KeywordsAntibiotic adaptation
antibiotic resistance
efflux pump
Staphylococcus aureus
Issue date2016
PublisherTaylor & Francis
CitationAbreu, Ana Cristina; Saavedra, Maria José; Simões, Lúcia C.; Simões, Manuel, Combinatorial approaches with selected phytochemicals to increase antibiotic efficacy against Staphylococcus aureus biofilms. Biofouling: The Journal of Bioadhesion and Biofilm Research, 32(9), 1103-1114, 2016
Abstract(s)Combinations of selected phytochemicals (reserpine, pyrrolidine, quinine, morin and quercetin) with antibiotics (ciprofloxacin, tetracycline and erythromycin) were tested on the prevention and control of Staphylococcus aureus biofilms. The phytochemicals were also studied for their ability to avoid antibiotic adaptation and to inhibit antibiotic efflux pumps. Morin, pyrrolidine and quercetin at subinhibitory concentrations had significant effects in biofilm prevention and/or control when applied alone and combined with antibiotics. Synergism between antibiotics and phytochemicals was found especially against biofilms of NorA overexpressing strain S. aureus SA1199B. This strain when growing with subinhibitory concentrations of ciprofloxacin developed increased tolerance to this antibiotic. However, this was successfully reversed by quinine and morin. In addition, reserpine and quercetin showed significant efflux pump inhibition. The overall results demonstrate the role of phytochemicals in co-therapies to promote more efficient treatments and decrease antimicrobial resistance to antibiotics, with substantial effects against S. aureus in both planktonic and biofilm states.
Publisher version
AccessOpen access
Appears in Collections:CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series

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