Please use this identifier to cite or link to this item: http://hdl.handle.net/1822/43072

TitleInfluence of nutritional and operational parameters on the production of butanol or 1,3-propanediol from glycerol by a mutant Clostridium pasteurianum
Author(s)Marusich, R.
Alves, M. M.
Rodrigues, L. R.
KeywordsClostridium pasteurianum
Random mutagenesis
Glycerol
Butanol
1,3-Propanediol
Issue date25-Jan-2017
PublisherElsevier
JournalNew Biotechnology
CitationMarusich, R.; Alves, M. Madalena; Rodrigues, Lígia R., Influence of nutritional and operational parameters on the production of butanol or 1,3-propanediol from glycerol by a mutant Clostridium pasteurianum. New Biotechnology, 34, 59-67, 2017
Abstract(s)Butanol and 1,3-propanediol (1,3-PDO) are simultaneously produced by Clostridium pasteurianum from glycerol. In this study, random chemical mutagenesis of C. pasteurianum DSM 525 was conducted to improve its tolerance to butanol. Selected nutritional and operational parameters were evaluated to identify strategies that favour the production of each metabolite. From those experiments, it was possible to isolate cells able to produce 22% more butanol than the parent strain in serum bottles. The supplementation of the culture medium with 2 mg l1 of iron increased the production of butanol by 163%, and the optimum inoculum age was found to be 12 h. Overall, the experiments conducted in bioreactor led to lower butanol titers than in serum bottles, which is attributed to the higher pressure present in the bottles. At pH 6.0, N2 sparging notoriously favoured the production of biomass and 1,3-PDO, while a lower pH (5.0) led to a higher butanol yield, although growth was negatively affected. The results herein gathered allowed the identification of specific conditions that favour the production of either butanol or 1,3-PDO. Furthermore, it was found that N2 sparging is a suitable strategy to maximize the titer, yield and productivity of 1,3-PDO using C. pasteurianum.
TypeArticle
URIhttp://hdl.handle.net/1822/43072
DOI10.1016/j.nbt.2016.03.002
ISSN1871-6784
e-ISSN1871-6784
Publisher versionhttp://www.journals.elsevier.com/new-biotechnology/
Peer-Reviewedyes
AccessOpen access
Appears in Collections:CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series

Files in This Item:
File Description SizeFormat 
document_38961_1.pdf1,33 MBAdobe PDFView/Open

Partilhe no FacebookPartilhe no TwitterPartilhe no DeliciousPartilhe no LinkedInPartilhe no DiggAdicionar ao Google BookmarksPartilhe no MySpacePartilhe no Orkut
Exporte no formato BibTex mendeley Exporte no formato Endnote Adicione ao seu ORCID