Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/42994
Registo completo
Campo DC | Valor | Idioma |
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dc.contributor.author | Almeida, Raquel Costa | por |
dc.contributor.author | Gasperini, Luca | por |
dc.contributor.author | Borges, João Paulo | por |
dc.contributor.author | Babo, Pedro Miguel Sousa | por |
dc.contributor.author | Rodrigues, Márcia T. | por |
dc.contributor.author | Mano, J. F. | por |
dc.contributor.author | Reis, R. L. | por |
dc.contributor.author | Gomes, Manuela E. | por |
dc.date.accessioned | 2016-11-11T11:54:11Z | - |
dc.date.issued | 2017 | - |
dc.date.submitted | 2016 | - |
dc.identifier.citation | Costa-Almeida R., Gasperini L., Borges J., Babo P. S., Rodrigues M. T., Mano J. F., Reis R. L., Gomes M. E. Microengineered Multicomponent Hydrogel Fibers: Combining Polyelectrolyte Complexation and Microfluidics, ACS Biomaterials Science & Engineering , doi:10.1021/acsbiomaterials.6b00331, 2017. | por |
dc.identifier.issn | 2373-9878 | por |
dc.identifier.uri | https://hdl.handle.net/1822/42994 | - |
dc.description.abstract | Fiber-based techniques hold great potential toward the development of structures that mimic the architecture of fibrous tissues, such as tendon. Microfluidics and polyelectrolyte complexation are among the most widely used techniques for the fabrication of fibrous structures. In this work, we combined both techniques to generate hydrogel fibers with a fibrillar-like structure. For this, either methacrylated hyaluronic acid (MA-HA) or chondroitin sulfate (MA-CS) were mixed with alginate (ALG), being all negatively charged polysaccharides, combined with chitosan (CHT), which is positively charged, and separately injected into a microfluidic device. Through a continuous injection into a coagulation bath and subsequent photo-cross-linking, we could obtain multi component hydrogel fibers, which exhibited smaller fibrils aligned in parallel, whenever CHT was present. The biological performance was assessed upon encapsulation and further culture of tendon cells. Overall, the reported process did not affect cell viability and cells were also able to maintain their main function of producing extracellular matrix up to 21 days in culture. In summary, we developed a novel class of photo-cross-linkable multicomponent hydrogel fibers than can act as bioactive modulators of cell behavior. | por |
dc.description.sponsorship | The authors thank to Hospital da Prelada (Porto, Portugal) for providing tendon tissue samples; to Portuguese funds through FCT Fundacao pars a Ciencia e a Tecnologia in the framework of FCT-POPH-FSE, the PhD and postdoctoral grants SFRH/BD/96593/2013 and SFRH/BPD/103604/2014 of R.C-A. and J.B., respectively, to the project RL3-TECT-NORTE-07-0124-FEDER-000020 cofinanced by ON.2 under the National Strategic Reference Framework (NSRF), through the European Regional Development Fund (ERDF) and the European Research Council for the project ComplexiTE grant agreement ERC-2012-ADG 20120216-321266. | por |
dc.language.iso | eng | por |
dc.publisher | ACS Publications | por |
dc.relation | SFRH/BD/96593/2013 | - |
dc.relation | SFRH/BPD/103604/2014 | - |
dc.relation | info:eu-repo/grantAgreement/EC/FP7/321266/EU | - |
dc.rights | restrictedAccess | por |
dc.subject | Chondroitin sulfate | por |
dc.subject | Fiber-based techniques | por |
dc.subject | Hyaluronic acid | por |
dc.subject | Microfluidics | por |
dc.subject | Polyelectrolyte complexation | por |
dc.subject | Tendon | por |
dc.subject | microfludics | por |
dc.title | Microengineered multicomponent hydrogel fibers: combining polyelectrolyte complexation and microfluidics | por |
dc.type | article | - |
dc.peerreviewed | yes | por |
dc.relation.publisherversion | http://pubs.acs.org/doi/abs/10.1021/acsbiomaterials.6b00331 | por |
dc.comments | http://3bs.uminho.pt/node/18881 | por |
sdum.publicationstatus | info:eu-repo/semantics/publishedVersion | por |
oaire.citationStartPage | 1322 | por |
oaire.citationEndPage | 1331 | por |
oaire.citationIssue | 7 | por |
oaire.citationTitle | ACS Biomaterials Science and Engineering | por |
oaire.citationVolume | 3 | por |
dc.date.updated | 2016-11-10T09:06:30Z | - |
dc.identifier.doi | 10.1021/acsbiomaterials.6b00331 | por |
dc.subject.wos | Science & Technology | por |
sdum.journal | ACS Biomaterials Science and Engineering | por |
Aparece nas coleções: | 3B’s - Artigos em revistas/Papers in scientific journals |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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18881-acsbiomaterials%2E6b00331.pdf Acesso restrito! | 7,02 MB | Adobe PDF | Ver/Abrir |