Utilize este identificador para referenciar este registo: https://hdl.handle.net/1822/42994

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Campo DCValorIdioma
dc.contributor.authorAlmeida, Raquel Costapor
dc.contributor.authorGasperini, Lucapor
dc.contributor.authorBorges, João Paulopor
dc.contributor.authorBabo, Pedro Miguel Sousapor
dc.contributor.authorRodrigues, Márcia T.por
dc.contributor.authorMano, J. F.por
dc.contributor.authorReis, R. L.por
dc.contributor.authorGomes, Manuela E.por
dc.date.accessioned2016-11-11T11:54:11Z-
dc.date.issued2017-
dc.date.submitted2016-
dc.identifier.citationCosta-Almeida R., Gasperini L., Borges J., Babo P. S., Rodrigues M. T., Mano J. F., Reis R. L., Gomes M. E. Microengineered Multicomponent Hydrogel Fibers: Combining Polyelectrolyte Complexation and Microfluidics, ACS Biomaterials Science & Engineering , doi:10.1021/acsbiomaterials.6b00331, 2017.por
dc.identifier.issn2373-9878por
dc.identifier.urihttps://hdl.handle.net/1822/42994-
dc.description.abstractFiber-based techniques hold great potential toward the development of structures that mimic the architecture of fibrous tissues, such as tendon. Microfluidics and polyelectrolyte complexation are among the most widely used techniques for the fabrication of fibrous structures. In this work, we combined both techniques to generate hydrogel fibers with a fibrillar-like structure. For this, either methacrylated hyaluronic acid (MA-HA) or chondroitin sulfate (MA-CS) were mixed with alginate (ALG), being all negatively charged polysaccharides, combined with chitosan (CHT), which is positively charged, and separately injected into a microfluidic device. Through a continuous injection into a coagulation bath and subsequent photo-cross-linking, we could obtain multi component hydrogel fibers, which exhibited smaller fibrils aligned in parallel, whenever CHT was present. The biological performance was assessed upon encapsulation and further culture of tendon cells. Overall, the reported process did not affect cell viability and cells were also able to maintain their main function of producing extracellular matrix up to 21 days in culture. In summary, we developed a novel class of photo-cross-linkable multicomponent hydrogel fibers than can act as bioactive modulators of cell behavior.por
dc.description.sponsorshipThe authors thank to Hospital da Prelada (Porto, Portugal) for providing tendon tissue samples; to Portuguese funds through FCT Fundacao pars a Ciencia e a Tecnologia in the framework of FCT-POPH-FSE, the PhD and postdoctoral grants SFRH/BD/96593/2013 and SFRH/BPD/103604/2014 of R.C-A. and J.B., respectively, to the project RL3-TECT-NORTE-07-0124-FEDER-000020 cofinanced by ON.2 under the National Strategic Reference Framework (NSRF), through the European Regional Development Fund (ERDF) and the European Research Council for the project ComplexiTE grant agreement ERC-2012-ADG 20120216-321266.por
dc.language.isoengpor
dc.publisherACS Publicationspor
dc.relationSFRH/BD/96593/2013-
dc.relationSFRH/BPD/103604/2014-
dc.relationinfo:eu-repo/grantAgreement/EC/FP7/321266/EU-
dc.rightsrestrictedAccesspor
dc.subjectChondroitin sulfatepor
dc.subjectFiber-based techniquespor
dc.subjectHyaluronic acidpor
dc.subjectMicrofluidicspor
dc.subjectPolyelectrolyte complexationpor
dc.subjectTendonpor
dc.subjectmicrofludicspor
dc.titleMicroengineered multicomponent hydrogel fibers: combining polyelectrolyte complexation and microfluidicspor
dc.typearticle-
dc.peerreviewedyespor
dc.relation.publisherversionhttp://pubs.acs.org/doi/abs/10.1021/acsbiomaterials.6b00331por
dc.commentshttp://3bs.uminho.pt/node/18881por
sdum.publicationstatusinfo:eu-repo/semantics/publishedVersionpor
oaire.citationStartPage1322por
oaire.citationEndPage1331por
oaire.citationIssue7por
oaire.citationTitleACS Biomaterials Science and Engineeringpor
oaire.citationVolume3por
dc.date.updated2016-11-10T09:06:30Z-
dc.identifier.doi10.1021/acsbiomaterials.6b00331por
dc.subject.wosScience & Technologypor
sdum.journalACS Biomaterials Science and Engineeringpor
Aparece nas coleções:3B’s - Artigos em revistas/Papers in scientific journals

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