Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/42954
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Campo DC | Valor | Idioma |
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dc.contributor.author | Ramos, Philippe Emmanuel Cruz | por |
dc.contributor.author | Cerqueira, Miguel Ângelo Parente Ribeiro | por |
dc.contributor.author | Cook, Michael T. | por |
dc.contributor.author | Bourbon, Ana Isabel Juncá Sottomayor Lisboa | por |
dc.contributor.author | Khutoryanskiy, Vitaliy V. | por |
dc.contributor.author | Charalampoulos, Dimitris | por |
dc.contributor.author | Teixeira, J. A. | por |
dc.contributor.author | Vicente, A. A. | por |
dc.date.accessioned | 2016-11-06T21:27:34Z | - |
dc.date.available | 2016-11-06T21:27:34Z | - |
dc.date.issued | 2016-12 | - |
dc.identifier.citation | Ramos, Philippe E.; Cerqueira, Miguel A.; Cook, Michael T.; Bourbon, Ana I.; Khutoryanskiy, Vitaliy V.; Charalampoulos, Dimitris; Teixeira, J. A.; Vicente, António A., Development of an immobilization system for in situ micronutrients release. Food Research International, 90, 121-132, 2016 | por |
dc.identifier.issn | 0963-9969 | por |
dc.identifier.uri | https://hdl.handle.net/1822/42954 | - |
dc.description.abstract | An immobilization system constituted by coated microcapsules was developed aiming at immobilizing probiotic bacteria capable of producing folate and release it in a sustained manner into the intestine. Despite no probiotic folate-producers have been immobilized so far, the system has been developed with this goal and this work reports its stability and ability to release folate under gastro-intestinal conditions. Microcapsules were made of alginate with three consecutive coatings of poly-l-lysine, sodium alginate and chitosan. Turbidity experiments showed a strong electrostatic interaction between these polymers. Fourier transform infrared spectroscopy (FTIR) and confocal analysis showed the stability of the coating materials when applied on the microcapsules, even after they were immersed in solutions simulating conditions in the stomach and small intestine (i.e. pH 2, 60 min and pH 7.2, 120 min, respectively). Coated microcapsules have an average diameter size ranged from 20 and 40 ?m, and swelled upon exposure to a neutral medium, without dissolution as showed by microscopy analyses. Release experiments proved the ability of the coated microcapsules to release folic acid, at different rates, depending on the applied coating. Release experiments showed that the first coating (?-PLL) is characterized by Fickian diffusion as the main release mechanism of folic acid. Fickian rate constant (kF) decreased with the number of consequent coatings, reflecting the decrease of predominance of Fick's behavior. Results showed that the developed coated microcapsules have suitable characteristics for encapsulation of folic acid aiming in situ release in the intestine. | por |
dc.description.sponsorship | The authors Philippe E. Ramos and Ana I. Bourbon, are recipient of fellowships from the Fundacão para a Ciência e Tecnologia, POPH-QREN and FSE (FCT, Portugal) through grants, SFRH/BD/80800/2012, SFRH/BD/73178/ 2010, respectively. The authors thank the FCT Strategic Project PEst-OE/EQB/ LA0023/2013 and the project “BioInd—Biotechnology and Bioengineering for improved Industrial and Agro-Food processes”, REF. NORTE-07-0124- FEDER-000028 co-funded by the Programa Operacional Regional do Norte (ON.2–O Novo Norte), QREN, FEDER. The authors would like to acknowledge to Graham Luke from Hopkins and Philip Lyle Microscopy Facilities (University of Reading) for assistance on confocal analysis. The authors also thank COST FA1001 and the European Network for Gastrointestinal Health Research (ENGIHR). | por |
dc.language.iso | eng | por |
dc.publisher | Elsevier 1 | por |
dc.relation | SFRH/BD/80800/2012 | por |
dc.relation | info:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBD%2F73178%2F2010/PT | por |
dc.relation | info:eu-repo/grantAgreement/FCT/3599-PPCDT/132966/PT | por |
dc.rights | openAccess | por |
dc.subject | Coated microcapsules | por |
dc.subject | Probiotics | por |
dc.subject | Folate | por |
dc.subject | Layer-by-layer | por |
dc.subject | Microcapsules | por |
dc.title | Development of an immobilization system for in situ micronutrients release | por |
dc.type | article | - |
dc.peerreviewed | yes | por |
dc.relation.publisherversion | http://www.journals.elsevier.com/food-research-international/ | por |
dc.comments | CEB46239 | por |
sdum.publicationstatus | info:eu-repo/semantics/publishedVersion | por |
oaire.citationStartPage | 121 | por |
oaire.citationEndPage | 132 | por |
oaire.citationConferencePlace | Netherlands | - |
oaire.citationTitle | Food Research International | por |
oaire.citationVolume | 90 | por |
dc.date.updated | 2016-11-05T18:53:58Z | - |
dc.identifier.doi | 10.1016/j.foodres.2016.10.050 | por |
dc.subject.wos | Science & Technology | por |
sdum.journal | Food Research International | por |
Aparece nas coleções: | CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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document_46239_1.pdf | 1,6 MB | Adobe PDF | Ver/Abrir |