Utilize este identificador para referenciar este registo: https://hdl.handle.net/1822/41904

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Campo DCValorIdioma
dc.contributor.authorMihaila, Silvia M.por
dc.contributor.authorResende, Margarida F.por
dc.contributor.authorReis, R. L.por
dc.contributor.authorGomes, Manuela E.por
dc.contributor.authorMarques, A. P.por
dc.date.accessioned2016-06-07T13:03:18Z-
dc.date.available2016-06-07T13:03:18Z-
dc.date.issued2017-
dc.identifier.citationMihaila S. M., Resende M., Reis R. L., Gomes M. E., Marques A. P. Interactive endothelial phenotype maintenance and osteogenic differentiation of adipose tissue stromal vascular fraction SSEA-4+ -derived cells, Journal of Tissue Engineering and Regenerative Medicine, doi:10.1002/term.2096, 2015por
dc.identifier.issn1932-7005por
dc.identifier.urihttps://hdl.handle.net/1822/41904-
dc.description"Version of Record online: 29 OCT 2015"por
dc.description.abstractBone formation relies on complex processes that require well-orchestrated interactions between several cell types, such as bone-forming cells (osteoblasts, OBs) and endothelial cells (ECs). Their co-culture has been proved relevant to mimicking specific features of the bone niche. Here we propose the co-culture of microvascular-like ECs and pre-OBs, both derived from the SSEA-4+ cell subpopulation from the stromal vascular fraction of human adipose tissue (SSEA-4+ hASCs), to define the conditions in which cells synergistically communicate to support the full differentiation of pre-OBs and maintenance of the EC phenotype. Co-cultures of different ratios of the two cell types were established and maintained for up to 21 days in standard endothelial maintenance (ENDO) and osteogenic differentiation (OST) media, as well as in a mixture of these (MIX). The osteogenic maturation of pre-OBs (ALP activity, OPN and OCN expression, calcium deposition), the evolution of EC numbers (CD31+ cells) and maintenance of the endothelial phenotype (CD31 and vWF expression, LDL uptake) were assessed throughout the culture time as a function of cell ratio and culture media. The results obtained demonstrate that EC number has a significant effect on the osteogenic differentiation of pre-OBs, depending on the medium used. While in ENDO medium the osteogenic differentiation was not observed, in the OST and MIX media it was attained at similar levels, except for the co-culture with a higher number of ECs in MIX medium. These findings demonstrate that the use of SSEA-4+ hASCs as a single-cell source is promising to attain 3D bone-like models with the potential to promote vascularized bone tissue regeneration.por
dc.description.sponsorshipThe authors are grateful to the Portuguese Foundation for Science and Technology (FCT) for personal grants SFRH/BD/ 42968/2008 through the MIT–Portugal Programme (SMM). The research leading to these results has received funding from the MIT/ECE/0047/2009 project, the European Union Seventh Framework Programme (Grant No. FP7/2007-2013, under Grant Agreement No. REGPOT-CT2012-316331-POLARIS) and the European Research Council (Advanced Grant No. ERC-2012- AdG_20120216-321266 for the project ComplexiTE).por
dc.language.isoengpor
dc.publisherJohn Wiley and Sonspor
dc.relationinfo:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBD%2F42968%2F2008/PT-
dc.relationinfo:eu-repo/grantAgreement/FCT/5876-PPCDTI/110812/PT-
dc.relationinfo:eu-repo/grantAgreement/EC/FP7/316331/EU-
dc.relationinfo:eu-repo/grantAgreement/EC/FP7/321266/EU-
dc.rightsopenAccesspor
dc.subjectCo-culturepor
dc.subjectSSEA-4-positive hASCspor
dc.subjectEndothelial cellspor
dc.subjectOsteoblast-like cellspor
dc.subjectBonepor
dc.subjectVascularizationpor
dc.titleInteractive endothelial phenotype maintenance and osteogenic differentiation of adipose tissue stromal vascular fraction SSEA-4+ -derived cellspor
dc.typearticle-
dc.peerreviewedyespor
dc.relation.publisherversionhttp://onlinelibrary.wiley.com/doi/10.1002/term.2096/fullpor
dc.commentshttp://3bs.uminho.pt/node/18708por
sdum.publicationstatusinfo:eu-repo/semantics/publishedVersionpor
oaire.citationStartPage1998por
oaire.citationEndPage2013por
oaire.citationIssue7por
oaire.citationTitleJournal of Tissue Engineering and Regenerative Medicinepor
oaire.citationVolume11por
dc.date.updated2016-06-06T10:29:07Z-
dc.identifier.doi10.1002/term.2096por
dc.identifier.pmid26510831por
dc.subject.wosScience & Technologypor
sdum.journalJournal of Tissue Engineering and Regenerative Medicinepor
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