Please use this identifier to cite or link to this item: http://hdl.handle.net/1822/41296

TitleLaccase immobilization on bacterial nanocellulose membranes: antimicrobial, kinetic and stability properties
Author(s)Sampaio, Liliana M. P.
Padrão, Jorge
Faria, Jorge
Silva, João P.
Silva, Carla J.
Dourado, Fernando
Zille, Andrea
KeywordsBacterial Cellulose
Laccase
Antimicrobial
Wound Dressing
Immobilization
Cytotoxicity
Issue date10-Jul-2016
PublisherElsevier
JournalCarbohydrate Polymers
CitationSampaio, Liliana M. P.; Padrão, Jorge; Faria, Jorge; Silva, João P.; Silva, Carla J.; Dourado, Fernando; Zille, Andrea, Laccase immobilization on bacterial nanocellulose membranes: antimicrobial, kinetic and stability properties. Carbohydrate Polymers, 145, 1-12, 2016
Abstract(s)This work studied the physical immobilization of a commercial laccase on bacterial nanocellulose (BNC) aiming to identify the laccase antibacterial properties suitable for wound dressings. Physico-chemical analysis demonstrates that the BNC structure is manly formed by pure crystalline I cellulose. The pH optimum and activation energy of free laccase depends on the substrate employed corresponding to pH 6, 7, 3 and 57, 22, 48 kJ mol1 for 2,6-dimethylphenol (DMP), catechol and 2,2 -azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), respectively. The Michaelis-Menten constant (Km) value for the immobilized laccase (0.77 mM) was found to be almost double of that of the free enzyme (0.42 mM). However, the specific activities of immobilized and free laccase are similar suggesting that the cage-like structure of BNC allows entrapped laccase to maintain some flexibility and favour substrate accessibility. The results clearly show the antimicrobial effect of laccase in Gram-positive (92%) and Gram-negative (26%) bacteria and cytotoxicity acceptable for wound dressing applications.
TypeArticle
URIhttp://hdl.handle.net/1822/41296
DOI10.1016/j.carbpol.2016.03.009
ISSN0144-8617
e-ISSN0144-8617
Publisher versionhttp://www.journals.elsevier.com/carbohydrate-polymers/
Peer-Reviewedyes
AccessOpen access
Appears in Collections:CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series

Files in This Item:
File Description SizeFormat 
document_37844_1.pdf1,76 MBAdobe PDFView/Open

Partilhe no FacebookPartilhe no TwitterPartilhe no DeliciousPartilhe no LinkedInPartilhe no DiggAdicionar ao Google BookmarksPartilhe no MySpacePartilhe no Orkut
Exporte no formato BibTex mendeley Exporte no formato Endnote Adicione ao seu ORCID