Please use this identifier to cite or link to this item:

TitlePoly(ester-urethane) scaffolds: effect of structure on properties and osteogenic activity of stem cells
Author(s)Kiziltay, A.
Marcos-Fernandez, A.
Roman, J. S.
Sousa, R. A.
Reis, R. L.
Hasirci, V.
Hasirci, N.
KeywordsBone marrow stem cells
Osteogenic activity
Issue dateMar-2015
JournalJournal of Tissue Engineering and Regenerative Medicine
CitationKiziltay, A., Marcos-Fernandez, A., San Roman, J., Sousa, R. A., Reis, R. L., Hasirci, V., & Hasirci, N. (2015). Poly(ester-urethane) scaffolds: effect of structure on properties and osteogenic activity of stem cells. Journal of Tissue Engineering and Regenerative Medicine, 9(8), 930-942. doi: 10.1002/term.1848
Abstract(s)The present study aimed to investigate the effect of structure (design and porosity) on the matrix stiffness and osteogenic activity of stem cells cultured on poly(ester-urethane) (PEU) scaffolds. Different three-dimensional (3D) forms of scaffold were prepared from lysine-based PEU using traditional salt-leaching and advanced bioplotting techniques. The resulting scaffolds were characterized by differential scanning calorimetry (DSC), thermogravimetric analysis (TGA), scanning electron microscopy (SEM), mercury porosimetry and mechanical testing. The scaffolds had various pore sizes with different designs, and all were thermally stable up to 300â °C. In vitrotests, carried out using rat bone marrow stem cells (BMSCs) for bone tissue engineering, demonstrated better viability and higher cell proliferation on bioplotted scaffolds compared to salt-leached ones, most probably due to their larger and interconnected pores and stiffer nature, as shown by higher compressive moduli, which were measured by compression testing. Similarly, SEM, von Kossa staining and EDX analyses indicated higher amounts of calcium deposition on bioplotted scaffolds during cell culture. It was concluded that the design with larger interconnected porosity and stiffness has an effect on the osteogenic activity of the stem cells.
Publisher version
AccessOpen access
Appears in Collections:3B’s - Artigos em revistas/Papers in scientific journals

Files in This Item:
File Description SizeFormat 
17899-term1848.pdf4,53 MBAdobe PDFView/Open

Partilhe no FacebookPartilhe no TwitterPartilhe no DeliciousPartilhe no LinkedInPartilhe no DiggAdicionar ao Google BookmarksPartilhe no MySpacePartilhe no Orkut
Exporte no formato BibTex mendeley Exporte no formato Endnote Adicione ao seu ORCID