Please use this identifier to cite or link to this item: http://hdl.handle.net/1822/40671

TitlePoly(ester-urethane) scaffolds: effect of structure on properties and osteogenic activity of stem cells
Author(s)Kiziltay, A.
Marcos-Fernandez, A.
Roman, J. S.
Sousa, R. A.
Reis, R. L.
Hasirci, V.
Hasirci, N.
KeywordsBone marrow stem cells
Osteogenic activity
Poly(ester-urethane)
Porosity
Scaffold
Stiffness
Issue dateMar-2015
PublisherWiley
JournalJournal of Tissue Engineering and Regenerative Medicine
CitationKiziltay, A., Marcos-Fernandez, A., San Roman, J., Sousa, R. A., Reis, R. L., Hasirci, V., & Hasirci, N. (2015). Poly(ester-urethane) scaffolds: effect of structure on properties and osteogenic activity of stem cells. Journal of Tissue Engineering and Regenerative Medicine, 9(8), 930-942. doi: 10.1002/term.1848
Abstract(s)The present study aimed to investigate the effect of structure (design and porosity) on the matrix stiffness and osteogenic activity of stem cells cultured on poly(ester-urethane) (PEU) scaffolds. Different three-dimensional (3D) forms of scaffold were prepared from lysine-based PEU using traditional salt-leaching and advanced bioplotting techniques. The resulting scaffolds were characterized by differential scanning calorimetry (DSC), thermogravimetric analysis (TGA), scanning electron microscopy (SEM), mercury porosimetry and mechanical testing. The scaffolds had various pore sizes with different designs, and all were thermally stable up to 300â °C. In vitrotests, carried out using rat bone marrow stem cells (BMSCs) for bone tissue engineering, demonstrated better viability and higher cell proliferation on bioplotted scaffolds compared to salt-leached ones, most probably due to their larger and interconnected pores and stiffer nature, as shown by higher compressive moduli, which were measured by compression testing. Similarly, SEM, von Kossa staining and EDX analyses indicated higher amounts of calcium deposition on bioplotted scaffolds during cell culture. It was concluded that the design with larger interconnected porosity and stiffness has an effect on the osteogenic activity of the stem cells.
TypeArticle
URIhttp://hdl.handle.net/1822/40671
DOI10.1002/term.1848
ISSN1932-7005
Publisher versionhttp://onlinelibrary.wiley.com/doi/10.1002/term.1848/full
Peer-Reviewedyes
AccessOpen access
Appears in Collections:3B’s - Artigos em revistas/Papers in scientific journals

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