Utilize este identificador para referenciar este registo: http://hdl.handle.net/1822/40670

TítuloIncorporation of antimicrobial peptides on functionalized cotton gauzes for medical applications
Autor(es)Gomes, A. P.
Mano, J. F.
Queiroz, J. A.
Gouveia, I. C.
Palavras-chaveAntimicrobial peptides
biocompatibility
Cys-LC-LL-37
Dermaseptin
hBD-1
human
Magainin 1
Wound-dressing
β-Defensin-1
DataAgo-2015
EditoraElsevier
RevistaCarbohydrate Polymers
CitaçãoGomes, A. P., Mano, J. F., Queiroz, J. A., & Gouveia, I. C. (2015). Incorporation of antimicrobial peptides on functionalized cotton gauzes for medical applications. Carbohydrate Polymers, 127, 451-461. doi: 10.1016/j.carbpol.2015.03.089
Resumo(s)A large group of low molecular weight natural compounds that exhibit antimicrobial activity has been isolated from animals and plants during the past two decades. Among them, peptides are the most widespread resulting in a new generation of antimicrobial agents with higher specific activity. In the present study we have developed a new strategy to obtain antimicrobial wound-dressings based on the incorporation of antimicrobial peptides into polyelectrolyte multilayer films built by the alternate deposition of polycation (chitosan) and polyanion (alginic acid sodium salt) over cotton gauzes. Energy dispersive X ray microanalysis technique was used to determine if antimicrobial peptides penetrated within the films. FTIR analysis was performed to assess the chemical linkages, and antimicrobial assays were performed with two strains: Staphylococcus aureus (Gram-positive bacterium) and Klebsiella pneumonia (Gram-negative bacterium). Results showed that all antimicrobial peptides used in this work have provided a higher antimicrobial effect (in the range of 4 log–6 log reduction) for both microorganisms, in comparison with the controls, and are non-cytotoxic to normal human dermal fibroblasts at the concentrations tested.
Tipoarticle
URIhttp://hdl.handle.net/1822/40670
DOI10.1016/j.carbpol.2015.03.089
ISSN0144-8617
Arbitragem científicayes
AcessorestrictedAccess
Aparece nas coleções:3B’s - Artigos em revistas/Papers in scientific journals


Partilhe no FacebookPartilhe no TwitterPartilhe no DeliciousPartilhe no LinkedInPartilhe no DiggAdicionar ao Google BookmarksPartilhe no MySpacePartilhe no Orkut
Exporte no formato BibTex mendeley Exporte no formato Endnote Adicione ao seu Currículo DeGóis