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https://hdl.handle.net/1822/40591
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Campo DC | Valor | Idioma |
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dc.contributor.author | Pinto, Vânia Cristina Gonçalves | por |
dc.contributor.author | Minas, Graça | por |
dc.contributor.author | Neves, Margarida Correia | por |
dc.date.accessioned | 2016-03-01T15:41:34Z | - |
dc.date.issued | 2015-02-26 | - |
dc.identifier.citation | Pinto, V. C., Minas, G., & Correia-Neves, M. (2015). PDMS biofunctionalization study for the development of a microfluidic device: Application to salivary cortisol. Paper presented at the Proceedings - 2015 IEEE 4th Portuguese Meeting on Bioengineering, ENBENG 2015. | - |
dc.identifier.isbn | 9781479982691 | por |
dc.identifier.uri | https://hdl.handle.net/1822/40591 | - |
dc.description | The authors acknowledge to Sofia Neves from ICVS for her help in the antibodies selection. | por |
dc.description.abstract | This paper presents the study of the PDMS (poly(dimethylsiloxane)) surface functionalization for the development of a microfluidic immunosensor that quantitatively analyss salivary cortisol by optical detection. The functionalization was performed using different antibodies immobilization methods on PDMS surface: (a) immobilization by passive adsorption on pristine PDMS; (b) silanization of PDMS surface with (3-aminopropyl)-triethoxysilane (APTES) to generate amino groups and posterior covalent immobilization of antibodies on APTES-PDMS using cross-linker glutaraldehyde (GA); (c) coating the PDMS surface with BSA to block non-specific protein adsorption, and then covalent bond of the protein A via GA. In this last approach, the antibodies were covalently immobilized to protein A due to its high affinity with the constant fraction (Fc) region of the antibodies. Atomic force microscope (AFM) and spectrophotometric analysis demonstrated that the immobilization method using protein A is more efficient since a higher roughness and uniformity on the PDMS surface and higher absorbance signals were obtained | por |
dc.description.sponsorship | This work is funded by FEDER funds through the "Eixo I do Programa Operacional Fatores de Competitividade (POFC) QREN, project references COMPETE: FCOMP-01-0124FEDER-020241 and by FCT – Fundação para a Ciência e a Tecnologia, project reference PTDC/EBB-EBI/120334/2010. V. C. Pinto thanks the FCT for the SFRH/BD/81526/2011 PhD grant. The authors acknowledge to Sofia Neves from ICVS for her help in the antibodies selection. | por |
dc.language.iso | eng | por |
dc.publisher | IEEE | por |
dc.relation | info:eu-repo/grantAgreement/FCT/5876-PPCDTI/120334/PT | por |
dc.relation | SFRH/BD/81526/2011 | por |
dc.rights | restrictedAccess | por |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | por |
dc.subject | Poly(dimethylsiloxane) (PDMS) | por |
dc.subject | Microfluidic immunosensor | por |
dc.subject | surface biofunctionalization | por |
dc.title | PDMS biofunctionalization study for the development of a microfluidic device: Application to salivary cortisol | por |
dc.type | conferencePaper | - |
dc.peerreviewed | yes | por |
dc.relation.publisherversion | http://dx.doi.org/10.1109/ENBENG.2015.7088852 | por |
sdum.publicationstatus | published | por |
oaire.citationConferenceDate | 26-28 February 2015 | por |
sdum.event.type | conference | por |
oaire.citationStartPage | 1 | por |
oaire.citationEndPage | 5 | por |
oaire.citationConferencePlace | Porto, Portugal | por |
oaire.citationTitle | IEEE 4th Portuguese Meeting on Bioengineering (ENBENG) | por |
dc.identifier.doi | 10.1109/ENBENG.2015.7088852 | por |
dc.subject.fos | Engenharia e Tecnologia::Outras Engenharias e Tecnologias | por |
dc.subject.wos | Science & Technology | por |
sdum.conferencePublication | IEEE 4th Portuguese Meeting on Bioengineering (ENBENG) | por |
sdum.bookTitle | 2015 IEEE 4TH PORTUGUESE MEETING ON BIOENGINEERING (ENBENG) | por |
Aparece nas coleções: | DEI - Artigos em atas de congressos internacionais |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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BioEng2015_IEEE_07088852_Vania.pdf Acesso restrito! | 817,06 kB | Adobe PDF | Ver/Abrir |
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