Please use this identifier to cite or link to this item: http://hdl.handle.net/1822/39513

TitleEffect of NaCl additive on solute-solvent interactions in aqueous polyethylene glycol-sodium sulfate two-phase systems
Author(s)Silva, N. M.
Ferreira, L.
Madeira, Pedro P.
Teixeira, J. A.
Uversky, Vladimir N.
Zaslavsky, Boris Y.
KeywordsAqueous two-phase system
Partitioning
Proteins
Solute-water interactions
Solvatochromic comparison method
Solvent properties
Issue date18-Dec-2015
PublisherElsevier
JournalJournal of Chromatography A
CitationSilva, N. M.; Ferreira, L.; Madeira, Pedro P.; Teixeira, J. A.; Uversky, Vladimir N.; Zaslavsky, Boris Y., Effect of NaCl additive on solute-solvent interactions in aqueous polyethylene glycol-sodium sulfate two-phase systems. Journal of Chromatography A, 1425, 51-61, 2015
Abstract(s)Abstract Partition behavior of eight small organic compounds and six proteins was examined in poly(ethylene glycol)-8000-sodium sulfate aqueous two-phase systems containing 0.215 M NaCl and 0.5 M osmolyte (sorbitol, sucrose, TMAO) and poly(ethylene glycol)-10000-sodium sulfate-0.215 M NaCl system, all in 0.01 M sodium phosphate buffer, pH 6.8. The differences between the solvent properties of the coexisting phases (solvent dipolarity/polarizability, hydrogen bond donor acidity, and hydrogen bond acceptor basicity) were characterized with solvatochromic dyes using the solvatochromic comparison method. Differences between the electrostatic properties of the phases were determined by analysis of partitioning of sodium salts of dinitrophenylated (DNP-) amino acids with aliphatic alkyl side-chain. The partition coefficients of all compounds examined (including proteins) were described in terms of solute-solvent interactions. The results obtained in the study show that solute-solvent interactions of nonionic organic compounds and proteins in polyethylene glycol-sodium sulfate aqueous two-phase system change in the presence of NaCl additive.
TypeArticle
URIhttp://hdl.handle.net/1822/39513
DOI10.1016/j.chroma.2015.11.019
ISSN0021-9673
e-ISSN0021-9673
Publisher versionhttp://www.journals.elsevier.com/journal-of-chromatography-a
Peer-Reviewedyes
AccessOpen access
Appears in Collections:CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series

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