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https://hdl.handle.net/1822/38722
Título: | Use of a polyphasic approach including MALDI-TOF MS for identification of Aspergillus section Flavi strains isolated from food commodities in Brazil |
Autor(es): | Silva, Fernanda Chaves da Chalfoun, Sara Maria Batista, Luis Roberto Santos, C. Lima, Nelson |
Palavras-chave: | Aspergillus section Flavi Food mycology MALDI-TOF MS Mycotoxin Polyphasic identification |
Data: | 2015 |
Editora: | Springer Verlag |
Revista: | Annals of Microbiology |
Citação: | Silva, Fernanda Chaves da; Chalfoun, Sara Maria; Batista, Luis Roberto; Santos, C.; Lima, Nelson, Use of a polyphasic approach including MALDI-TOF MS for identification of Aspergillus section Flavi strains isolated from food commodities in Brazil. Annals of Microbiology, 65(4), 2119-2129, 2015 |
Resumo(s): | Brazil is one the largest producers and exporters of food commodities in the world. The evaluation of fungi capable of spoilage and the production mycotoxins in these commodities is an important issue that can be of help in bioeconomic development. The present work aimed to identify fungi of the genus Aspergillus section Flavi isolated from different food commodities in Brazil. Thirty-five fungal isolates belonging to the section Flavi were identified and characterised. Different classic phenotypic and genotypic methodologies were used, as well as a novel approach based on proteomic profiles produced by matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry (MALDI-TOF MS). Type or reference strains for each taxonomic group were included in this study. Three isolates that presented discordant identification patterns were further analysed using the internal transcribed spacer (ITS) region and calmodulin gene sequences. The data obtained from the phenotypic and spectral analyses divide the isolates into three groups, corresponding to taxa closely related to Aspergillus flavus, Aspergillus parasiticus, and Aspergillus tamarii. Final polyphasic fungal identification was achieved by joining data from molecular analyses, classical morphology, and biochemical and proteomic profiles generated by MALDI-TOF MS. |
Tipo: | Artigo |
URI: | https://hdl.handle.net/1822/38722 |
DOI: | 10.1007/s13213-015-1050-0 |
ISSN: | 1590-4261 |
e-ISSN: | 1869-2044 |
Versão da editora: | http://www.springer.com/life+sciences/microbiology/journal/13213 |
Arbitragem científica: | yes |
Acesso: | Acesso aberto |
Aparece nas coleções: | CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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document_19845_1.pdf | 866,15 kB | Adobe PDF | Ver/Abrir |