Please use this identifier to cite or link to this item: http://hdl.handle.net/1822/38722

TitleUse of a polyphasic approach including MALDI-TOF MS for identification of Aspergillus section Flavi strains isolated from food commodities in Brazil
Author(s)Silva, Fernanda Chaves da
Chalfoun, Sara Maria
Batista, Luis Roberto
Santos, C.
Lima, Nelson
KeywordsAspergillus section Flavi
Food mycology
MALDI-TOF MS
Mycotoxin
Polyphasic identification
Issue date2015
PublisherSpringer Verlag
JournalAnnals of Microbiology
CitationSilva, Fernanda Chaves da; Chalfoun, Sara Maria; Batista, Luis Roberto; Santos, C.; Lima, Nelson, Use of a polyphasic approach including MALDI-TOF MS for identification of Aspergillus section Flavi strains isolated from food commodities in Brazil. Annals of Microbiology, 65(4), 2119-2129, 2015
Abstract(s)Brazil is one the largest producers and exporters of food commodities in the world. The evaluation of fungi capable of spoilage and the production mycotoxins in these commodities is an important issue that can be of help in bioeconomic development. The present work aimed to identify fungi of the genus Aspergillus section Flavi isolated from different food commodities in Brazil. Thirty-five fungal isolates belonging to the section Flavi were identified and characterised. Different classic phenotypic and genotypic methodologies were used, as well as a novel approach based on proteomic profiles produced by matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry (MALDI-TOF MS). Type or reference strains for each taxonomic group were included in this study. Three isolates that presented discordant identification patterns were further analysed using the internal transcribed spacer (ITS) region and calmodulin gene sequences. The data obtained from the phenotypic and spectral analyses divide the isolates into three groups, corresponding to taxa closely related to Aspergillus flavus, Aspergillus parasiticus, and Aspergillus tamarii. Final polyphasic fungal identification was achieved by joining data from molecular analyses, classical morphology, and biochemical and proteomic profiles generated by MALDI-TOF MS.
TypeArticle
URIhttp://hdl.handle.net/1822/38722
DOI10.1007/s13213-015-1050-0
ISSN1590-4261
e-ISSN1869-2044
Publisher versionhttp://www.springer.com/life+sciences/microbiology/journal/13213
Peer-Reviewedyes
AccessOpen access
Appears in Collections:CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series

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