Utilize este identificador para referenciar este registo: http://hdl.handle.net/1822/38567

TítuloIn vitro chondrogenic commitment of human Wharton's jelly stem cells by co-culture with human articular chondrocytes.
AutorPereira, R. C.
Costa-Pinto, A. R.
Frias, A. M.
Neves, N. M.
Azevedo, Helena S.
Reis, R. L.
Palavras-chaveCartilage
Chondrogenic differentiation
Co-culture
Human articular chondrocytes
Human stem cells
DataSet-2015
EditoraWiley
CitaçãoPereira R. C., Costa-Pinto A. R., Frias A. M., Neves N. M., Azevedo H. S., Reis R. L. In vitro chondrogenic commitment of human Wharton's jelly stem cells by co-culture with human articular chondrocytes., Journal of Tissue Engineering and Regenerative Medicine, Vol. DOI: 10.1002/term.2085, 2015
ResumoWharton's jelly stem cells (WJSCs) are a potential source of transplantable stem cells in cartilage-regenerative strategies, due to their highly proliferative and multilineage differentiation capacity. We hypothesized that a non-direct co-culture system with human articular chondrocytes (hACs) could enhance the potential chondrogenic phenotype of hWJSCs during the expansion phase compared to those expanded in monoculture conditions. Primary hWJSCs were cultured in the bottom of a multiwell plate separated by a porous transwell membrane insert seeded with hACs. No statistically significant differences in hWJSCs duplication number were observed under either of the culture conditions during the expansion phase. hWJSCs under co-culture conditions show upregulations of collagen type I and II, COMP, TGFβ1 and aggrecan, as well as of the main cartilage transcription factor, SOX9, when compared to those cultured in the absence of chondrocytes. Chondrogenic differentiation of hWJSCs, previously expanded in co-culture and monoculture conditions, was evaluated for each cellular passage using the micromass culture model. Cells expanded in co-culture showed higher accumulation of glycosaminoglycans (GAGs) compared to cells in monoculture, and immunohistochemistry for localization of collagen type I revealed a strong detection signal when hWJSCs were expanded under monoculture conditions. In contrast, type II collagen was detected when cells were expanded under co-culture conditions, where numerous round-shaped cell clusters were observed. Using a micromass differentiation model, hWJSCs, previously exposed to soluble factors secreted by hACs, were able to express higher levels of chondrogenic genes with deposition of cartilage extracellular matrix components, suggesting their use as an alternative cell source for treating degenerated cartilage.
Tipoarticle
URIhttp://hdl.handle.net/1822/38567
DOI10.1002/term.2085
ISSN1932-7005
Versão da editorahttp://onlinelibrary.wiley.com/doi/10.1002/term.2085/abstract
Arbitragem científicayes
AcessoopenAccess
Aparece nas coleções:3B’s - Artigos em revistas/Papers in scientific journals

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