Please use this identifier to cite or link to this item: http://hdl.handle.net/1822/38498

TitleSaloplastic membranes as green devices for soft tissue regeneration
Author(s)Costa, Rui R.
Mano, J. F.
Costa, A. M. S.
Caridade, S. G.
KeywordsSaloplastics
Soft tissues
Issue dateSep-2015
PublisherMary Ann Liebert
JournalTissue Engineering. Part A
CitationCosta R. R., Mano J. F., Costa A. M. S., Caridade S. G. Saloplastic Membranes as Green Devices for Soft Tissue Regeneration, Tissue Engineering Part A, Vol. 21, Issue S1, pp. S159, doi:10.1089/ten.tea.2015.5000.abstracts, 2015
Abstract(s)Implantable devices must exhibit mechanical properties similar to native tissues to promote appropriate cellular behavior and regeneration. Herein, we report a new membrane manufacture method based on the synthesis of polyelectrolyte complexes (PECs) that exhibit saloplasticity, i.e. variable physical-chemistry using salt as a plasticizer. This is a Green Chemistry approach, as PECs generate structures that are stabilized solely by reversible electrostatic interactions, avoiding the use of harmful crosslinkers completely. Furthermore, natural polyelectrolytes - chitosan and alginate - were used. Upon mixing them, membranes were obtained by drying the PECs at 37ºC, yielding compact PECs without resorting to organicsolvents. The plasticizing effect of salt after synthesis was shown by measuring tensile mechanical properties, which were lower when samples were immersed in high ionic strength solutions.Salt was also used during membrane synthesis in different quan- tities (0 M, 0.15 M and 0.5 M in NaCl) yielding structures with no significant differences in morphology and degradation (around 15% after 3 months in lysozyme). However, swelling was higher (about 10x) when synthesized in the presence of salt. In vitro cell studies using L929 fibroblasts showed that cells adhered and proliferated preferentially in membranes fabricated in the presence of salt (i.e. the membranes with lower tensile strength). Structures with physical-chemical properties controlled with precision open a path to tissue engineering strategies depending on fine tuning mechanical properties and cellular adhesion simply by changing ionic strength during membrane manufacture
TypeAbstract
URIhttp://hdl.handle.net/1822/38498
ISSN1937-3341
Publisher versionhttp://online.liebertpub.com/toc/tea/21/S1
Peer-Reviewedyes
AccessOpen access
Appears in Collections:3B’s - Resumos em livros de atas de conferências - indexados no ISI Web of Science

Files in This Item:
File Description SizeFormat 
18530-TEng_21_S1.pdf3,37 MBAdobe PDFView/Open

Partilhe no FacebookPartilhe no TwitterPartilhe no DeliciousPartilhe no LinkedInPartilhe no DiggAdicionar ao Google BookmarksPartilhe no MySpacePartilhe no Orkut
Exporte no formato BibTex mendeley Exporte no formato Endnote Adicione ao seu ORCID