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TitleOsteogenic differentiation of adipose stem cells by endothelial cells co-culture within liquified capsules
Author(s)Correia, Clara R.
Pirraco, Rogério P.
Cerqueira, M. T.
Marques, A. P.
Reis, R. L.
Mano, J. F.
Endothelial cells
Osteogenic differentiation
Stem cells
Issue dateAug-2015
PublisherMary Ann Liebert
CitationCorreia C. R., Pirraco R. P., Cerqueira M. T., Marques A. P., Reis R. L., Mano J. F. Osteogenic Differentiation of Adipose Stem Cells by Endothelial Cells Co-culture Within Liquified Capsules, Tissue Engineering Part A, Vol. 21, Issue S1, pp. S1-S413, doi:10.1089/ten.tea.2015.5000.abstracts, 2015
Abstract(s)Inspired by the native co-existence of multiple cell types and from the concept of deconstructing the stem cell niche, we propose a co-encapsulation strategy within liquified capsules. The present team has already proven the application of liquified capsules as bioencapsulation systems1. Here, we intend to use the optimized system towards osteogenic differentiation. Capsules encapsulating adipose stem cells alone (MONO-capsules) or in co-culture with endothelial cells (CO-capsules) were maintained in endothelial medium with or without osteogenic differentiation factors. The suitability of the capsules for living stem and endothelial cells encapsulation was demonstrated by MTS and DNA assays. The osteogenic differentiation was assessed by quantifying the deposition of calcium and the activity of ALP up to 21 days. CO capsules had an enhanced osteogenic differentiation, even when cultured in the absence of osteogenic factors. Furthermore, osteopontin and CD31 could be detected, which respectively indicate that osteogenic differentiation had occurred and endothelial cells maintained their phenotype. An enhanced osteogenic differentiation by co-encapsulation was also confirmed by the upregulation of osteogenic markers (BMP-2, RUNX2, BSP) while the expression of angiogenic markers (VEGF, vWF, CD31) revealed the presence of endothelial cells. The proposed capsules can also act as a growth factor release system upon implantation, as showed by VEGF and BMP-2 quantification. These findings demonstrate that the co-encapsulation of stem and endothelial cells within liquified injectable capsules provides a promising strategy for bone tissue engineering.  
Publisher version
AccessOpen access
Appears in Collections:3B’s - Resumos em livros de atas de conferências - indexados no ISI Web of Science

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