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TitleCo-assembly, spatiotemporal control and morphogenesis of a hybrid protein-peptide system
Author(s)Inostroza-Brito, K. E.
Collin, E.
Siton-Mendelson, O.
Smith, K. H.
Monge-Marcet, A.
Ferreira, D. S.
Rodríguez, R. P.
Alonso, M.
Rodríguez-Cabello, J. C.
Reis, R. L.
Sagués, F.
Botto, L.
Bitton, R.
Azevedo, Helena S.
Mata, A.
KeywordsBioinspired materials
Biomedical materials
Molecular self-assembly
Issue dateSep-2015
PublisherNature Publishing Group
JournalNature Chemistry
CitationInostroza-Brito K. E., Collin E., Siton-Mendelson O., Smith K. H., Monge-Marcet A., Ferreira D. S., Rodríguez R. P., Alonso M., Rodríguez-Cabello J. C., Reis R. L., Sagués F., Botto L., Bitton R., Azevedo H. S., Mata A. Co-assembly, spatiotemporal control and morphogenesis of a hybrid protein-peptide system, Nature Chemistry, Vol. 7, Issue 11, pp. 897–904, doi:doi:10.1038/nchem.2349, 2015
Abstract(s)Controlling molecular interactions between bioinspired molecules can enable the development of new materials with higher complexity and innovative properties. Here we report on a dynamic system that emerges from the conformational modification of an elastin-like protein by peptide amphiphiles and with the capacity to access, and be maintained in, non-equilibrium for substantial periods of time. The system enables the formation of a robust membrane that displays controlled assembly and disassembly capabilities, adhesion and sealing to surfaces, self-healing and the capability to undergo morphogenesis into tubular structures with high spatiotemporal control. We use advanced microscopy along with turbidity and spectroscopic measurements to investigate the mechanism of assembly and its relation to the distinctive membrane architecture and the resulting dynamic properties. Using cell-culture experiments with endothelial and adipose-derived stem cells, we demonstrate the potential of this system to generate complex bioactive scaffolds for applications such as tissue engineering.
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Appears in Collections:3B’s - Artigos em revistas/Papers in scientific journals

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