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https://hdl.handle.net/1822/37777
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Campo DC | Valor | Idioma |
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dc.contributor.author | Nogueira, Eugénia Sofia Costa | por |
dc.contributor.author | Lager, Franck | por |
dc.contributor.author | Le Roux, Delphine | por |
dc.contributor.author | Nogueira, Patrícia | por |
dc.contributor.author | Freitas, Jaime | por |
dc.contributor.author | Charvet, Celine | por |
dc.contributor.author | Renault, Gilles | por |
dc.contributor.author | Loureiro, Ana | por |
dc.contributor.author | Almeida, Catarina R. | por |
dc.contributor.author | Ohradanova-Repic, Anna | por |
dc.contributor.author | Machacek, Christian | por |
dc.contributor.author | Bernardes, Gonçalo J. L. | por |
dc.contributor.author | Moreira, Alexandra | por |
dc.contributor.author | Stockinger, Hannes | por |
dc.contributor.author | Burnet, Michael | por |
dc.contributor.author | Carmo, Alexandre M. | por |
dc.contributor.author | Gomes, Andreia C | por |
dc.contributor.author | Preto, Ana | por |
dc.contributor.author | Bismuth, Georges | por |
dc.contributor.author | Cavaco-Paulo, Artur | por |
dc.date.accessioned | 2015-10-26T16:53:36Z | - |
dc.date.available | 2015-10-26T16:53:36Z | - |
dc.date.issued | 2015-03-18 | - |
dc.identifier.citation | Nogueira, E.; Lager, Franck; Le Roux, Delphine; Nogueira, Patrícia; Freitas, Jaime; Charvet, Celine; Renault, Gilles; Loureiro, A.; Almeida, Catarina R.; Ohradanova-Repic, Anna; Machacek, Christian; Bernardes, Gonçalo J. L.; Moreira, Alexandra; Stockinger, Hannes; Burnet, Michael; Carmo, Alexandre M.; Gomes, A. C.; Preto, A.; Bismuth, Georges; Paulo, Artur Cavaco, Enhancing methotrexate tolerance with folate tagged liposomes in arthritic mice. Journal of Biomedical Nanotechnology, 11(12), 2243-2252, 2015 | por |
dc.identifier.issn | 1550-7033 | por |
dc.identifier.uri | https://hdl.handle.net/1822/37777 | - |
dc.description.abstract | Methotrexate is the first line of treatment of rheumatoid arthritis. Since many patients become unresponsive to methotrexate treatment, only very expensive biological therapies are effective and increased methotrexate tolerance strategies need to be identified. Here we propose the encapsulation of methotrexate in a new liposomal formulation using a hydrophobic fragment of surfactant protein conjugated to a linker and folate to enhance their tolerance and efficacy. In this study we aim to evaluate the efficiency of this system to treat rheumatoid arthritis, by targeting folate receptor present at the surface of activated macrophages, key effector cells in this pathology. The specificity of our liposomal formulation to target folate receptor was investigated both in vitro as in vivo using a mouse model of arthritis (collagen-induced arthritis in DBA/1J mice strain). In both systems, the liposomal constructs were shown to be highly specific and efficient in targeting folate receptor . These liposomal formulations also significantly increase the clinical benefit of the encapsulated methotrexate in vivo in arthritic mice, together with reduced expression of CD39 and CD73 ectonucleotidases by joint-infiltrating macrophages. Thus, our formulation might be a promising cost effective way to treat rheumatoid arthritis and delay or reduce methotrexate intolerance. | por |
dc.description.sponsorship | Eugenia Nogueira (SFRH/BD/81269/2011), Ana Loureiro (SFRH/BD/81479/2011) and Catarina Almeida (SFRH/BPD/48533/2008) hold scholarships from Fundacao para a Ciencia e a Tecnologia (FCT). Goncalo J. L. Bernardes is a Royal Society University Research Fellow at the Department of Chemistry, University of Cambridge and an Investigador FCT at the Instituto de Medicina Molecular, Faculdade de Medicina da Universidade de Lisboa. This study was funded by the European Union Seventh Framework Programme (FP7/2007-2013) under grant agreement NMP4-LA-2009-228827 NANOFOL. This study was also supported by FEDER through POFC-COMPETE, by national funds from FCT through the project PEst-C/BIA/UI4050/2014 and the strategic funding of ID/BIO/04469/2013 unit. We thank the Immuno-haemotherapy Department of Hospital de Sao Joao (Porto, Portugal) for providing buffy coats from healthy volunteers. We also thank Noemy Gueriba for her technical assistance in various experiments. | por |
dc.language.iso | eng | por |
dc.publisher | American Scientific Publishers | por |
dc.relation | info:eu-repo/grantAgreement/FCT/FARH/SFRH%2FBD%2F81269%2F2011/PT | por |
dc.relation | info:eu-repo/grantAgreement/FCT/FARH/SFRH%2FBD%2F81479%2F2011/PT | por |
dc.relation | info:eu-repo/grantAgreement/FCT/FARH/SFRH%2FBPD%2F48533%2F2008/PT | por |
dc.relation | FP7/2007-2013 | por |
dc.relation | NMP4-LA-2009-228827 | por |
dc.relation | info:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/PEst-OE%2FBIA%2FUI4050%2F2014/PT | por |
dc.relation | ID/BIO/04469/2013 | por |
dc.rights | openAccess | por |
dc.subject | Rheumatoid Arthritis | por |
dc.subject | Methotrexate | por |
dc.subject | Macrophages | por |
dc.subject | Folate Receptor | por |
dc.subject | Liposomes | por |
dc.title | Enhancing methotrexate tolerance with folate tagged liposomes in arthritic mice | por |
dc.type | article | - |
dc.peerreviewed | yes | por |
dc.relation.publisherversion | http://www.aspbs.com/jbn.html | por |
dc.comments | CEB22389 | por |
sdum.publicationstatus | published | por |
oaire.citationStartPage | 2243 | por |
oaire.citationEndPage | 2252 | por |
oaire.citationIssue | 12 | por |
oaire.citationConferencePlace | United States | - |
oaire.citationTitle | Journal of Biomedical Nanotechnology | por |
oaire.citationVolume | 11 | por |
dc.date.updated | 2015-09-22T21:50:16Z | - |
dc.identifier.eissn | 1550-7041 | - |
dc.identifier.doi | 10.1166/jbn.2015.2170 | por |
dc.identifier.pmid | 26510317 | por |
dc.subject.wos | Science & Technology | por |
sdum.journal | Journal of Biomedical Nanotechnology | por |
Aparece nas coleções: | CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series CBMA - Artigos/Papers DBio - Artigos/Papers |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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document_22389_1.pdf | 8,37 MB | Adobe PDF | Ver/Abrir |