Please use this identifier to cite or link to this item: http://hdl.handle.net/1822/37621

TitleAnalysis of partitioning of organic compounds and proteins in aqueous polyethylene glycol-sodium sulfate aqueous two-phase systems in terms of solute-solvent interactions
Author(s)Silva, N. M.
Ferreira, L.
Madeira, Pedro
Teixeira, J. A.
Uversky, Vladimir N.
Zaslavsky, Boris Y.
KeywordsAqueous two-phase system
Partitioning
Proteins
Solutewater interactions
Solvatochromic comparison method
Solvent properties
Issue date9-Oct-2015
PublisherElsevier
JournalJournal of Chromatography A
CitationSilva, N. M.; Ferreira, L.; Madeira, Pedro P.; Teixeira, J. A.; Uversky, Vladimir N.; Zaslavsky, Boris Y., Analysis of partitioning of organic compounds and proteins in aqueous polyethylene glycol-sodium sulfate aqueous two-phase systems in terms of solutesolvent interactions. Journal of Chromatography A, 1415, 1-10, 2015
Abstract(s)Partition behavior of nine small organic compounds and six proteins was examined in poly(ethylene glycol)-8000-sodium sulfate aqueous two-phase systems containing 0.5 M osmolyte (sorbitol, sucrose, trehalose, TMAO) and poly(ethylene glycol)-10000-sodium sulfate system, all in 0.01 M sodium phosphate buffer, pH 6.8. The differences between the solvent properties of the coexisting phases (solvent dipolarity/polarizability, hydrogen bond donor acidity, and hydrogen bond acceptor basicity) were characterized with solvatochromic dyes using the solvatochromic comparison method. Differences between the electrostatic properties of the phases were determined by analysis of partitioning of sodium salts of dinitrophenylated (DNP-) amino acids with aliphatic alkyl side-chain. It was found out that the partition coefficient of all compounds examined (including proteins) may be described in terms of solutesolvent interactions. The results obtained in the study show that solutesolvent interactions of nonionic organic compounds and proteins in polyethylene glycol-sodium sulfate aqueous two-phase system differ from those in polyethylene glycol-dextran system.
TypeArticle
URIhttp://hdl.handle.net/1822/37621
DOI10.1016/j.chroma.2015.08.053
ISSN0021-9673
e-ISSN0021-9673
Publisher versionhttp://www.journals.elsevier.com/journal-of-chromatography-a
Peer-Reviewedyes
AccessOpen access
Appears in Collections:CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series

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