Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/37129
Registo completo
Campo DC | Valor | Idioma |
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dc.contributor.author | Padrão, Jorge | por |
dc.contributor.author | Machado, Raul | por |
dc.contributor.author | Casal, Margarida | por |
dc.contributor.author | Lanceros-Méndez, S. | por |
dc.contributor.author | Rodrigues, L. R. | por |
dc.contributor.author | Dourado, Fernando | por |
dc.contributor.author | Sencadas, V. | por |
dc.date.accessioned | 2015-09-11T10:13:05Z | - |
dc.date.available | 2015-09-11T10:13:05Z | - |
dc.date.issued | 2015-11 | - |
dc.date.submitted | 2015-02 | - |
dc.identifier.citation | Padrão, Jorge; Machado, Raul; Casal, Margarida; Lanceros-Méndez, Senentxu; Rodrigues, L. R.; Dourado, Fernando; Sencadas, Vitor, Antibacterial performance of bovine lactoferrin-fish gelatine electrospun membranes. International Journal of Biological Macromolecules, 81, 608-614, 2015 | por |
dc.identifier.issn | 0141-8130 | por |
dc.identifier.uri | https://hdl.handle.net/1822/37129 | - |
dc.description.abstract | The increase of antibiotic resistant microorganisms urged the development and synthesis of novel antimicrobial biomaterials to be employed in a broad range of applications, ranging from food packaging to medical devices. This work describes the production and characterization of a protein-based electrospun fibrous membranes bearing antimicrobial properties. Its composition is exclusively comprised of proteins, with fish gelatine as structural matrix and bovine lactoferrin (bLF) as the active antimicrobial agent. The bLF bactericidal effect was determined against clinical isolates of Escherichia coli and Staphylococcus aureus through microdilution assays. Two distinctive methods were used to incorporate bLF into the fish gelatine nanofibres: (i) as a filler in the electrospinning formulation with concentrations of 2, 5 and 10 (wt%), and cross-linked with glutaraldehyde vapour, in order to achieve stability in aqueous solution; and (ii) through adsorption in a solution with 40 mg mL1 bLF. Fourier transform infrared spectroscopy analysis showed that the structure of both proteins remained intact through the electrospinning blending and cross-linking procedure. Remarkable antibacterial properties were obtained with membranes containing 5% and 10% bLF with a bacterial reduction of approximately 90% and 100%, respectively. | por |
dc.description.sponsorship | Portuguese Foundation for Science and Technology (FCT) Strategic Project PEst-OE/EQB/LA0023/ 2013 UID/BIA/04050/2013, UID/BIO/04469/2013, and for the research grants funding through POPH/FSE. FEDER funds through the “Programa Operacional Fatores de Competitividade – COMPETE” and by national funds arranged by FCT FCT, project ref- erences PEST-C/FIS/UI607/2014, Matepro – Optimizing Materials and Processes”, ref. NORTE-07-0124-FEDER-000037”, RECI/BBB- EBI/0179/2012 (FCOMP-01-0124-FEDER-027462) and the project “BioInd - Biotechnology and Bioengineering for improved Industrial and Agro-Food processes”, REF. NORTE-07-0124-FEDER-000028, co-funded by the “Programa Operacional Regional do Norte” (ON.2 – O Novo Norte), under the “Quadro de Referência Estratégico Nacional” (QREN), through the “Fundo Europeu de Desenvolvimento Regional” (FEDER). COST Action MP1206 “Electrospun Nano-fibres for bio inspired composite materials and innovative industrial applications”. | por |
dc.language.iso | eng | por |
dc.publisher | Elsevier B.V. | por |
dc.rights | openAccess | por |
dc.subject | Electrospinning | por |
dc.subject | Fish gelatine | por |
dc.subject | Bovine lactoferrin membranes | por |
dc.subject | Bactericidal | por |
dc.subject | Proteins | por |
dc.title | Antibacterial performance of bovine lactoferrin-fish gelatine electrospun membranes | por |
dc.type | article | - |
dc.peerreviewed | yes | por |
dc.relation.publisherversion | http://www.sciencedirect.com/science/article/pii/S0141813015005942 | por |
dc.comments | CEB22357 | por |
sdum.publicationstatus | published | por |
oaire.citationStartPage | 608 | por |
oaire.citationEndPage | 614 | por |
oaire.citationConferencePlace | Netherlands | - |
oaire.citationTitle | International Journal of Biological Macromolecules | por |
oaire.citationVolume | 81 | por |
dc.date.updated | 2015-09-07T13:35:39Z | - |
dc.identifier.doi | 10.1016/j.ijbiomac.2015.08.047 | por |
dc.identifier.pmid | 26314908 | por |
dc.subject.wos | Science & Technology | por |
sdum.journal | International Journal of Biological Macromolecules | por |
Aparece nas coleções: | CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series DBio - Artigos/Papers |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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document_22357_1.pdf | 1,43 MB | Adobe PDF | Ver/Abrir |