Please use this identifier to cite or link to this item: http://hdl.handle.net/1822/35302

TitleContribution of PRS3, RPB4 and ZWF1 to the resistance of industrial Saccharomyces cerevisiae CCUG53310 and PE-2 strains to lignocellulosic hydrolysate-derived inhibitors
Author(s)Cunha, J. T.
Aguiar, Tatiana Quinta
Romaní, Aloia
Oliveira, Carla Cristina Marques de
Domingues, Lucília
KeywordsSecond generation bioethanol
Lignocellulose-derived inhibitors
Industrial Saccharomyces cerevisiae
PRS3, RPB4 and ZWF1
Expression and overexpression analysis
Issue dateSep-2015
PublisherElsevier B.V.
JournalBioresource Technology
CitationCunha, J. T.; Aguiar, T. Q.; Romaní, Aloia; Oliveira, Carla Cristina Marques de; Domingues, Lucília, Contribution of PRS3, RPB4 and ZWF1 to the resistance of industrial Saccharomyces cerevisiae CCUG53310 and PE-2 strains to lignocellulosic hydrolysate-derived inhibitors. Bioresource Technology, 191, 7-16, 2015
Abstract(s)PRS3, RPB4 and ZWF1 were previously identified as key genes for yeast tolerance to lignocellulose-derived inhibitors. To better understand their contribution to yeast resistance to the multiple stresses occurring during lignocellulosic hydrolysate fermentations, we overexpressed these genes in two industrial Saccharomyces cerevisiae strains, CCUG53310 and PE-2, and evaluated their impact on the fermentation of Eucalyptus globulus wood and corn cob hydrolysates. PRS3 overexpression improved the fermentation rate (up to 32%) and productivity (up to 48%) in different hydrolysates. ZWF1 and RPB4 overexpression did not improve the fermentation performance, but their increased expression in the presence of acetic acid, furfural and hydroxymethylfurfural was found to contribute to yeast adaptation to these inhibitors. This study expands our understanding about the molecular mechanisms involved in industrial yeast tolerance to the stresses occurring during lignocellulosic bioethanol production and highlights the importance of selecting appropriate strain backgrounds/hydrolysates combinations when addressing further improvement of these processes.
TypeArticle
URIhttp://hdl.handle.net/1822/35302
DOI10.1016/j.biortech.2015.05.006
ISSN0960-8524
e-ISSN0960-8524
Publisher versionhttp://www.sciencedirect.com/science/article/pii/S0960852415006707
Peer-Reviewedyes
AccessOpen access
Appears in Collections:CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series

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