Please use this identifier to cite or link to this item: http://hdl.handle.net/1822/35207

TitleDetection and quantification of fluconazole within Candida glabrata biofilms
Author(s)Rodrigues, Célia F.
Silva, Sónia Carina
Azeredo, Joana
Henriques, Mariana
KeywordsCandida
Biofilm matrix
Antifungal
Method
Resistance
Issue date23-Jan-2015
PublisherKluwer
JournalMycopathologia
CitationRodrigues, Célia F.; Silva, Sónia Carina; Azeredo, Joana; Henriques, Mariana, Detection and quantification of fluconazole within Candida glabrata biofilms. Mycopathologia, 179(5-6), 391-395, 2015
Abstract(s)Candida infections are often associated with biofilms and consequent high resistance to most common drugs (e.g. azoles). These resistance mechanisms are not only associated with the biofilm yeast physiology, but also with the presence of a diffusional barrier imposed by the biofilm matrix; however, the real biochemical role of the biofilm components remains very unclear. So, in order to further clarify this issue, we intend to determine, for the first time, fluconazole in biofilms within both supernatants and matrices. Candida biofilms were formed in the presence of fluconazole, and it was recovered from both supernatant and matrix cell-free fractions. Then, high-pressure liquid chromatography was used to identify and quantify the amount of drug that was present in the two fractions. Moreover, this study also showed that the presence of fluconazole in both fractions indicated that the drug administrated did not completely reach the cells, so this phenomena can easily be associated with lower biofilm susceptibility, since the drug administered did not completely reach the cells.
TypeArticle
URIhttp://hdl.handle.net/1822/35207
DOI10.1007/s11046-015-9862-9
ISSN1573-0832
e-ISSN0301-486X
Peer-Reviewedyes
AccessOpen access
Appears in Collections:CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series

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