Please use this identifier to cite or link to this item: http://hdl.handle.net/1822/35025

TitleNatural assembly of platelet lysate-loaded nanocarriers into enriched 3D hydrogels for cartilage regeneration
Author(s)Santo, Vitor E.
Popa, Elena Geta
Mano, J. F.
Gomes, Manuela E.
Reis, R. L.
KeywordsNanoparticles
platelet lysates
Self-assembly hydrogel
Tissue engineering
Self-assembly
Hydrogel
Issue dateMar-2015
PublisherElsevier
JournalActa Biomaterialia
Abstract(s)The role of Platelet Lysates (PLs) as a source of growth factors (GFs) and as main element of threedimensional (3D) hydrogels has been previously described. However, the resulting hydrogels usually suffer from high degree of contraction, limiting their usefulness. This work describes the development of a stable biomimetic 3D hydrogel structure based on PLs, through the spontaneous assembling of a high concentration of chitosan-chondroitin sulfate nanoparticles (CH/CS NPs) with PLs loaded by adsorption. The interactions between the NPs and the lysates resemble the ones observed in the extracellular matrix (ECM) native environment between glycosaminoglycans and ECM proteins. In vitro release studies were carried out focusing on the quantification of PDGF-BB and TGF-b1 GFs. Human adipose derived stem cells (hASCs) were entrapped in these 3D hydrogels and cultured in vitro under chondrogenic stimulus, in order to assess their potential use for cartilage regeneration. Histological, immunohistological and gene expression analysis demonstrated that the PL-assembled constructs entrapping hASCs exhibited results similar to the positive control (hASCS cultured in pellets), concerning the levels of collagen II expression and immunolocalization of collagen type I and II and aggrecan. Moreover, the deposition of new cartilage ECM was detected by alcian blue and safranin-O positive stainings. This work demonstrates the potential of PLs to act simultaneously as a source/carrier of GFs and as a 3D structure of support, through the application of a â â bottom-upâ â approach involving the assembly of NPs, resulting in an enriched construct for cartilage regeneration applications.
TypeArticle
URIhttp://hdl.handle.net/1822/35025
DOI10.1016/j.actbio.2015.03.015
ISSN1742-7061
Publisher versionhttp://dx.doi.org/10.1016/j.actbio.2015.03.015
Peer-Reviewedyes
AccessOpen access
Appears in Collections:3B’s - Artigos em revistas/Papers in scientific journals

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