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https://hdl.handle.net/1822/34592
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Campo DC | Valor | Idioma |
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dc.contributor.author | Rodrigues, Joana Lúcia Lima Correia | por |
dc.contributor.author | Araújo, R. G. | por |
dc.contributor.author | Prather, Kristala L. J. | por |
dc.contributor.author | Kluskens, Leon | por |
dc.contributor.author | Rodrigues, L. R. | por |
dc.date.accessioned | 2015-03-27T14:43:15Z | - |
dc.date.available | 2015-03-27T14:43:15Z | - |
dc.date.issued | 2015 | - |
dc.identifier.citation | Rodrigues, Joana Lúcia; Araújo, R. G.; Prather, Kristala L. J.; Kluskens, Leon; Rodrigues, L. R., Heterologous production of caffeic acid from tyrosine in Escherichia coli. Enzyme and Microbial Technology, 71, 36-44, 2015 | por |
dc.identifier.issn | 0141-0229 | por |
dc.identifier.uri | https://hdl.handle.net/1822/34592 | - |
dc.description.abstract | Caffeic acid is a plant secondary metabolite and its biological synthesis has attracted increased attention due to its beneficial effects on human health. In this study, Escherichia coli was engineered for the production of caffeic acid using tyrosine as the initial precursor of the pathway. The pathway design included tyrosine ammonia lyase (TAL) from Rhodotorula glutinis to convert tyrosine to p-coumaric acid and 4-coumarate 3-hydroxylase (C3H) from Saccharothrix espanaensis or cytochrome P450 CYP199A2 from Rhodopseudomonas palustris to convert p-coumaric acid to caffeic acid. The genes were codon-optimized and different combinations of plasmids were used to improve the titer of caffeic acid. TAL was able to efficiently convert 3 mM of tyrosine to p-coumaric acid with the highest production obtained being 2.62 mM (472 mg/L). CYP199A2 exhibited higher catalytic activity towards p-coumaric acid than C3H. The highest caffeic acid production obtained using TAL and CYP199A2 and TAL and C3H was 1.56 mM (280 mg/L) and 1 mM (180 mg/L), respectively. This is the first study that shows caffeic acid production using CYP199A2 and tyrosine as the initial precursor. This study suggests the possibility of further producing more complex plant secondary metabolites like flavonoids and curcuminoids. | por |
dc.description.sponsorship | This work was supported by FEDER funds through the COMPETE and ON2 program and through National funds of FCT in the scope of the project FCOMP-01-0124-FEDER-027462, PEst-OE/EQB/LA0023/2013, NORTE-07-0124-FEDER-000028 and NORTE-07-0124-FEDER-000027. Financial support for this work was provided by FCT grant SFRH/BD/51187/2010 and SYNBIOBACTHER project (PTDC/EBB-BIO/102863/2008). | por |
dc.language.iso | eng | por |
dc.publisher | Elsevier 1 | por |
dc.rights | openAccess | por |
dc.subject | Tyrosine | por |
dc.subject | p-Coumaric acid | por |
dc.subject | Caffeic acid | por |
dc.subject | E. coli | por |
dc.subject | Biosynthesis | por |
dc.subject | Synthetic biology | por |
dc.title | Heterologous production of caffeic acid from tyrosine in Escherichia coli | por |
dc.type | article | - |
dc.peerreviewed | yes | por |
dc.relation.publisherversion | http://www.journals.elsevier.com/enzyme-and-microbial-technology/ | por |
dc.comments | CEB19706 | por |
sdum.publicationstatus | published | por |
oaire.citationStartPage | 36 | por |
oaire.citationEndPage | 44 | por |
oaire.citationConferencePlace | Netherlands | - |
oaire.citationTitle | Enzyme and microbial technology | por |
oaire.citationVolume | 71 | por |
dc.date.updated | 2015-03-10T22:34:11Z | - |
dc.identifier.eissn | 0141-0229 | - |
dc.identifier.doi | 10.1016/j.enzmictec.2015.01.001 | por |
dc.identifier.pmid | 25765308 | por |
dc.subject.wos | Science & Technology | por |
sdum.journal | Enzyme and microbial technology | por |
Aparece nas coleções: | CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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document_19706_1.pdf | 841,57 kB | Adobe PDF | Ver/Abrir |