Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/32877
Título: | Dioctadecyldimethylammonium: monoolein nanocarriers for efficient in vitro gene silencing |
Autor(es): | Oliveira, Ana Cristina Norberto Gonçalves Martens, Thomas Raemdonck, Koen Adati, Renata Feitosa, Eloi Botelho, C. M. Gomes, Andreia C Braeckmans, Kevin Real Oliveira, M. Elisabete C.D. |
Palavras-chave: | Counterion Gene silencing Liposomes Monoolein siRNA delivery |
Data: | 7-Abr-2014 |
Editora: | American Chemical Society |
Revista: | ACS Applied Materials and Interfaces |
Citação: | Oliveira, Ana; Martens, Thomas; Raemdonck, Koen; Adati, Renata; Feitosa, Eloi; Botelho, C. M.; Gomes, Andreia; Braeckmans, Kevin; Real Oliveira, Maria Elisabete, Dioctadecyldimethylammonium:monoolein nanocarriers for efficient in vitro gene silencing. ACS Applied Materials & Interfaces, 6(9), 6977-6989, 2014 |
Resumo(s): | This study describes a novel liposomal formulation for siRNA delivery, based on the mixture of the neutral lipid monoolein (MO) and cationic lipids of the dioctadecyldimethylammonium (DODA) family. The cationic lipids dioctadecyldimethylammonium bromide (DODAB) and chloride (DODAC) were compared in order to identify which one will most efficiently induce gene silencing. MO has a fluidizing effect on DODAC and DODAB liposomes, although it was more homogeneously distributed in DODAC bilayers. All MO-based liposomal formulations were able to efficiently encapsulate siRNA. Stable lipoplexes of small size (100-160 nm) with a positive surface charge (>+45 mV) were formed. A more uniform MO incorporation in DODAC:MO may explain an increase of the fusogenic potential of these liposomes. The siRNA-lipoplexes were readily internalized by human nonsmall cell lung carcinoma (H1299) cells, in an energy dependent process. DODAB:MO nanocarriers showed a higher internalization efficiency in comparison to DODAC:MO lipoplexes, and were also more efficient in promoting gene silencing. MO had a similar gene silencing ability as the commonly used helper lipid 1,2-dioleyl-3-phosphatidylethanolamine (DOPE), but with much lower cytotoxicity. Taking in consideration all the results presented, DODAB:MO liposomes are the most promising tested formulation for systemic siRNA delivery. |
Tipo: | Artigo |
URI: | https://hdl.handle.net/1822/32877 |
DOI: | 10.1021/am500793y |
ISSN: | 1944-8244 |
e-ISSN: | 1944-8252 |
Arbitragem científica: | yes |
Acesso: | Acesso aberto |
Aparece nas coleções: | CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series CBMA - Artigos/Papers CDF - FAMO - Artigos/Papers (with refereeing) DBio - Artigos/Papers |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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document_17701_1.pdf | 4,92 MB | Adobe PDF | Ver/Abrir |