Please use this identifier to cite or link to this item: http://hdl.handle.net/1822/31942

TitleSonochemically-induced spectral shift as a probe of green fluorescent protein release from nano capsules
Author(s)Shimanovich, Ulyana
Munder, A.
Azóia, Nuno G.
Paulo, Artur Cavaco
Gruzman, A.
Knowles, T. P. J.
Gedanken, Aharon
Issue date31-Jan-2014
PublisherRoyal Society of Chemistry
JournalRSC Advances
CitationShimanovich, U.; Munder, A.; Azoia, Nuno G.; Paulo, Artur Cavaco; Gruzman, A.; Knowles, T. P. J.; Gedanken, A., Sonochemically-induced spectral shift as a probe of green fluorescent protein release from nano capsules. RSC Advances, 4(20), 10303-10309, 2014
Abstract(s)Encapsulation in the form of micro and nanocapsules is an attractive route for controlling the delivery and release of active proteins and peptides. Many approaches exist to probe the morphology of such capsules as well as their mechanisms of formation. By contrast, the release of proteins from such components in a complex biological environment has been challenging to probe directly. In this paper we show that the spectral differences between green fluorescent protein (GFP) in capsules and in its free form can be used to monitor in situ the release of the protein from the confinement of capsules. These findings represent a new route towards engineering the spectral characteristics of GFP through physical rather than chemical means. We demonstrate the use of GFP protein capsules for monitoring in real time the release of protein in live cells by exposing rat L6 myotubes to protein capsules. The GFP spheres with a blue fluorescent signal dissociate inside the L6 myotubes to individual GFP molecules with a change in fluorescent signal from blue to green. These sensitive spectral characteristics enabled us to resolve the dissociation of capsules inside the cells in both time and space. We discuss the implications of our results for quantifying parameters crucial for the delivery of proteins in biological environments.
TypeArticle
URIhttp://hdl.handle.net/1822/31942
DOI10.1039/C3RA47915C
ISSN2046-2069
e-ISSN2046-2069
Publisher versionhttp://pubs.rsc.org/en/journals/journalissues/ra
Peer-Reviewedyes
AccessOpen access
Appears in Collections:CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series

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