Utilize este identificador para referenciar este registo: https://hdl.handle.net/1822/31642

Registo completo
Campo DCValorIdioma
dc.contributor.authorZhang, Yunleipor
dc.contributor.authorNicolau, Anapor
dc.contributor.authorLima, Cristóvão F.por
dc.contributor.authorRodrigues, L. R.por
dc.date.accessioned2014-12-04T18:00:38Z-
dc.date.available2014-12-04T18:00:38Z-
dc.date.issued2014-
dc.identifier.citationZhang, Y.; Nicolau, Ana; Lima, C. F.; Rodrigues, L. R., Bovine lactoferrin induces cell cycle arrest and inhibits mTOR signaling in breast cancer cells. Nutrition and Cancer, 66(8), 1371-1385, 2014por
dc.identifier.issn1532-7914por
dc.identifier.urihttps://hdl.handle.net/1822/31642-
dc.description.abstractLactoferrin (LF) is predominantly found in mammalian secretions with recognized anticancer potential, although the mechanisms involved in such activity are still unclear. Here, the stability, internalization, and cytotoxicity of bovine LF (bLF) and its variants were tested against a panel of breast cancer cells. bLF was found to be very stable under incubation with cells and also able to internalize them, although most of the protein remained in the culture medium. Furthermore, bLF (up to 30 μM) inhibited the growth of breast cancer cells (T-47D, MDA-MB-231, Hs578T, and MCF-7) in a higher extent than in the normal counterpart cell line (MCF-10-2A), thus suggesting its selectivity. Regarding its variants, only the iron-saturated protein showed a higher activity compared with the commercial bLF. bLF growth inhibitory activity was associated with the induction of cell cycle arrest, but not with apoptosis. Moreover, exposure to bLF increased the cells phospho-AMPKα levels and decreased both phospho threonine mammalian target of rapamycin (mTOR) and total mTOR levels, indicating a novel mechanism of action through its ability to induce nutrient/energy-related stress. This study disclosed important findings to better understand the mechanisms underlying the bLF effects on breast cancer cell lines, which could be valuable for novel advances in the cancer research field.por
dc.description.sponsorshipWe acknowledge the financial support from Erasmus Mundus External Cooperation window (Bridging the Gap), the Strategic Projects PEst-OE/EQB/LA0023/2013 and PEst-OE/AGR/UI4033/2014, as well as the Project RECI/BBB-EBI/0179/2012 (FCOMP-01-0124-FEDER-027462), all funded by Fundacao para a Ciencia e a Tecnologia.por
dc.language.isoengpor
dc.publisherLawrence Erlbaum Associates Inc.por
dc.rightsrestrictedAccesspor
dc.subjectLactoferrinpor
dc.subjectBreast cancerpor
dc.subjectmTorpor
dc.titleBovine lactoferrin induces cell cycle arrest and inhibits mTOR signaling in breast cancer cellspor
dc.typearticle-
dc.peerreviewedyespor
dc.commentsCEB18057por
sdum.publicationstatuspublishedpor
oaire.citationStartPage1371por
oaire.citationEndPage1385por
oaire.citationIssue8por
oaire.citationConferencePlaceUnited States-
oaire.citationTitleNutrition and Cancerpor
oaire.citationVolume66por
dc.date.updated2014-11-28T17:45:49Z-
dc.identifier.eissn0163-5581-
dc.identifier.doi10.1080/01635581.2014.956260por
dc.identifier.pmid25356800por
dc.subject.wosScience & Technologypor
sdum.journalNutrition and Cancerpor
Aparece nas coleções:CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series

Ficheiros deste registo:
Ficheiro Descrição TamanhoFormato 
document_18057_1.pdf
Acesso restrito!
982,67 kBAdobe PDFVer/Abrir

Partilhe no FacebookPartilhe no TwitterPartilhe no DeliciousPartilhe no LinkedInPartilhe no DiggAdicionar ao Google BookmarksPartilhe no MySpacePartilhe no Orkut
Exporte no formato BibTex mendeley Exporte no formato Endnote Adicione ao seu ORCID