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https://hdl.handle.net/1822/31641
Título: | Optimization of xylanase production by filamentous fungi in solid state fermentation and scale-up to horizontal tube bioreactor |
Autor(es): | Pérez-Rodríguez, N. Oliveira, Felisbela Pérez-Bibbins, B. Belo, Isabel Torrado Agrasar, A. Domínguez, José Manuel |
Palavras-chave: | Corncob Xylanases Solid-state fermentation Aspergillus niger |
Data: | 2014 |
Editora: | Springer |
Revista: | Applied Biochemistry and Biotechnology |
Citação: | Pérez-Rodríguez, N.; Oliveira, Felisbela; Pérez-Bibbins, B.; Belo, Isabel; Torrado Agrasar, A.; Domínguez, J. M. , Optimization of xylanase production by filamentous fungi in solid state fermentation and scale-up to horizontal tube bioreactor. Applied Biochemistry and Biotechnology, 173, 803-825, 2014 |
Resumo(s): | Five microorganisms, namely Aspergillus niger CECT 2700, A. niger CECT 2915, A. niger CECT 2088, Aspergillus terreus CECT 2808, and Rhizopus stolonifer CECT 2344, were grown on corncob to produce cell wall polysaccharide-degrading enzymes, mainly xylanases, by solid-state fermentation (SSF). A. niger CECT 2700 produced the highest amount of xylanases of 504±7 U/g dry corncob (dcc) after 3 days of fermentation. The optimization of the culture broth (5.0 g/L NaNO3, 1.3 g/L (NH4)2SO4, 4.5 g/L KH2PO4, and 3 g/L yeast extract) and operational conditions (5 g of bed loading, using an initial substrate to moistening medium of 1:3.6 (w/v)) allowed increasing the predicted maximal xylanase activity up to 2,452.7 U/g dcc. However, different pretreatments of materials, including destarching, autoclaving, microwave, and alkaline treatments, were detrimental. Finally, the process was successfully established in a laboratory-scale horizontal tube biore- actor, achieving the highest xylanase activity (2,926 U/g dcc) at a flow rate of 0.2 L/min. The result showed an overall 5.8-fold increase in xylanase activity after optimization of culture media, operational conditions, and scale-up. |
Tipo: | Artigo |
URI: | https://hdl.handle.net/1822/31641 |
DOI: | 10.1007/s12010-014-0895-1 |
ISSN: | 0273-2289 |
e-ISSN: | 0273-2289 |
Versão da editora: | http://link.springer.com/journal/12010 |
Arbitragem científica: | yes |
Acesso: | Acesso aberto |
Aparece nas coleções: | CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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document_18063_1.pdf | 1,24 MB | Adobe PDF | Ver/Abrir |