Please use this identifier to cite or link to this item: http://hdl.handle.net/1822/30983

TitleTailored freestanding multilayered membranes based on chitosan and alginate
Author(s)Silva, Joana M.
Duarte, Ana Rita C.
Caridade, S. G.
Picart, Catherine
Reis, R. L.
Mano, J. F.
KeywordsAlginate
Chitosan
Diffusion
Issue dateSep-2014
PublisherACS Publications
JournalBiomacromolecules
Abstract(s)Engineering metabolically demanding tissues requires the supply of nutrients, oxygen, and removal of metabolic byproducts, as well as adequate mechanical properties. In this work, we propose the development of chitosan (CHIT)/alginate (ALG) freestanding membranes fabricated by layer-by-layer (LbL) assembly. CHIT/ALG membranes were cross-linked with genipin at a concentration of 1 mg· mL−1 or 5 mg·mL−1. Mass transport properties of glucose and oxygen were evaluated on the freestanding membranes. The diffusion of glucose and oxygen decreases with increasing cross-linking concentration. Mechanical properties were also evaluated in physiological-simulated conditions. Increasing cross-linking density leads to an increase of storage modulus, Young modulus, and ultimate tensile strength, but to a decrease in the maximum hydrostatic pressure. The in vitro biological performance demonstrates that cross-linked films are more favorable for cell adhesion. This work demonstrates the versatility and feasibility of LbL assembly to generate nanostructured constructs with tunable permeability, mechanical, and biological properties.
TypeArticle
URIhttp://hdl.handle.net/1822/30983
DOI10.1021/bm501156v
ISSN1525-7797
Publisher versionhttp://pubs.acs.org/doi/abs/10.1021/bm501156v
Peer-Reviewedyes
AccessRestricted access (UMinho)
Appears in Collections:3B’s - Artigos em revistas/Papers in scientific journals

Files in This Item:
File Description SizeFormat 
18165-bm501156v.pdf
  Restricted access
7,42 MBAdobe PDFView/Open

Partilhe no FacebookPartilhe no TwitterPartilhe no DeliciousPartilhe no LinkedInPartilhe no DiggAdicionar ao Google BookmarksPartilhe no MySpacePartilhe no Orkut
Exporte no formato BibTex mendeley Exporte no formato Endnote Adicione ao seu ORCID