Utilize este identificador para referenciar este registo: http://hdl.handle.net/1822/30303

TítuloHyaluronic acid/poly-L-lysine bilayered silica nanoparticles enhance the osteogenic differentiation of human mesenchymal stem cells
Autor(es)Amorim, Sara
Martins, Albino
Neves, N. M.
Reis, R. L.
Pires, R. A.
Palavras-chaveHyaluronic acid
Layer-by-layer
Osteogenesis
Data2014
EditoraRoyal Society of Chemistry
RevistaJournal of materials chemistry B
Resumo(s)Herein, we evaluate the influence of a PLL–HA bilayer on the surface of silica nanoparticles on their capacity to induce the osteogenic differentiation of human bone marrow stem cells (hBMSCs), as a function of their concentration (50 μg mL−1, 25 μg mL−1 and 12.5 μg mL−1). To this purpose, we synthesized silica nanoparticles (diameter of 250 nm; ζ-potential of −25 mV) that were coated with PLL–HA (diameter of 560 nm; ζ-potential of −35 mV). The cell viability, cell proliferation, protein quantification (i.e. MTS, DNA and ALP, respectively) and gene expression (of osteogenesis-related genes: ALP, osteocalcin, collagen type I, bone sialoprotein, Runx-2, osteopontin and osterix) were monitored, for 21 days. We observed the overexpression of most of the tested osteogenic transcripts in the hBMSCs cultured with SiO2–PLL–HA, at concentrations of 25 μg mL−1 and 12.5 μg mL−1. These results indicate that the proposed nanoparticles temporarily improve the osteogenic differentiation of hBMSCs at low nanoparticle concentrations.
Tipoarticle
DescriçãoFirst published online 15 Aug 2014
URIhttp://hdl.handle.net/1822/30303
DOI10.1039/c4tb01071j
ISSN2050-750X
Versão da editorahttp://pubs.rsc.org/en/content/articlepdf/2014/tb/c4tb01071j
Arbitragem científicayes
AcessoopenAccess
Aparece nas coleções:3B’s - Artigos em revistas/Papers in scientific journals

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