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https://hdl.handle.net/1822/3006
Título: | Ontogenic variations in n-alkanes during somatic embryogenesis of flax (Linum usitatissimum L.) |
Autor(es): | Cunha, Ana Ferreira, Manuel Fernandes |
Palavras-chave: | Linum usitatissimum L. Flax Lipids N-alkanes Tissue culture Somatic embryogenesis |
Data: | Mai-2001 |
Editora: | Elsevier 1 |
Revista: | Plant Science |
Citação: | "Plant Science". ISSN 0168-9452. 160:6 (2001) 1137-1143. |
Resumo(s): | Hypocotyl segments of flax seedlings germinated in vitro, were used to induce indirect somatic embryogenesis on solid medium. The composition and distribution of n-alkanes in flax tissues collected at different developmental stages were studied by GC and GC-MS. During induction and development of callus from hypocotyl tissues a decrease in the percentage of total lipids was observed. In all types of tissue sampled - hypocotyl segments used as primary explants, hypocotyl segments with differentiating calli at the cut ends, embryogenic and non-embryogenic calli and somatic embryos - a skewed-normal distribution of n-alkanes with a low mass range (C13-C21) were found. The highest content of n-alkanes occurred in the primary hypocotyl explants and in the early stages of callus development. Longer carbon chain n-alkanes were observed only in the mature or differentiated tissues of hypocotyls and somatic embryos. Although the n-alkane contents decreased with time, in somatic embryos and calli, a significantly lower n-alkane content was observed in embryogenic calli when compared to non-embryogenic calli independent of the time in culture. These results suggest the utilisation of n-alkanes for heterotrophic cellular growth as well as its mobilisation from embryogenic calli to developing somatic embryos |
Tipo: | Artigo |
URI: | https://hdl.handle.net/1822/3006 |
DOI: | 10.1016/S0168-9452(01)00358-2 |
ISSN: | 0168-9452 |
Versão da editora: | http://www.elsevier.com/wps/find/journaldescription.cws_home/506030/description#description |
Arbitragem científica: | yes |
Acesso: | Acesso aberto |
Aparece nas coleções: | DBio - Artigos/Papers |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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postprint4.pdf | Documento completo - postprint | 198,2 kB | Adobe PDF | Ver/Abrir |