Utilize este identificador para referenciar este registo: https://hdl.handle.net/1822/3006

TítuloOntogenic variations in n-alkanes during somatic embryogenesis of flax (Linum usitatissimum L.)
Autor(es)Cunha, Ana
Ferreira, Manuel Fernandes
Palavras-chaveLinum usitatissimum L.
Flax
Lipids
N-alkanes
Tissue culture
Somatic embryogenesis
DataMai-2001
EditoraElsevier 1
RevistaPlant Science
Citação"Plant Science". ISSN 0168-9452. 160:6 (2001) 1137-1143.
Resumo(s)Hypocotyl segments of flax seedlings germinated in vitro, were used to induce indirect somatic embryogenesis on solid medium. The composition and distribution of n-alkanes in flax tissues collected at different developmental stages were studied by GC and GC-MS. During induction and development of callus from hypocotyl tissues a decrease in the percentage of total lipids was observed. In all types of tissue sampled - hypocotyl segments used as primary explants, hypocotyl segments with differentiating calli at the cut ends, embryogenic and non-embryogenic calli and somatic embryos - a skewed-normal distribution of n-alkanes with a low mass range (C13-C21) were found. The highest content of n-alkanes occurred in the primary hypocotyl explants and in the early stages of callus development. Longer carbon chain n-alkanes were observed only in the mature or differentiated tissues of hypocotyls and somatic embryos. Although the n-alkane contents decreased with time, in somatic embryos and calli, a significantly lower n-alkane content was observed in embryogenic calli when compared to non-embryogenic calli independent of the time in culture. These results suggest the utilisation of n-alkanes for heterotrophic cellular growth as well as its mobilisation from embryogenic calli to developing somatic embryos
TipoArtigo
URIhttps://hdl.handle.net/1822/3006
DOI10.1016/S0168-9452(01)00358-2
ISSN0168-9452
Versão da editorahttp://www.elsevier.com/wps/find/journaldescription.cws_home/506030/description#description
Arbitragem científicayes
AcessoAcesso aberto
Aparece nas coleções:DBio - Artigos/Papers

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