Utilize este identificador para referenciar este registo: http://hdl.handle.net/1822/28412

TítuloUnderstanding the role of growth factors in modulating stem cell tenogenesis
Autor(es)Gonçalves, A. I.
Rodrigues, Márcia T.
Lee, S. J.
Atala, A.
Yoo, James
Reis, R. L.
Gomes, Manuela E.
Palavras-chaveGrowth factors
Stem cells
Tenogenesis
DataDez-2013
EditoraPlos One
RevistaPLoS ONE
Resumo(s)Current treatments for tendon injuries often fail to fully restore joint biomechanics leading to the recurrence of symptoms, and thus resulting in a significant health problem with a relevant social impact worldwide. Cell-based approaches involving the use of stem cells might enable tailoring a successful tendon regeneration outcome. As growth factors (GFs) powerfully regulate the cell biological response, their exogenous addition can further stimulate stem cells into the tenogenic lineage, which might eventually depend on stem cells source. In the present study we investigate the tenogenic differentiation potential of human- amniotic fluid stem cells (hAFSCs) and adipose-derived stem cells (hASCs) with several GFs associated to tendon development and healing; namely, EGF, bFGF, PDGF-BB and TGF-β1. Stem cells response to biochemical stimuli was studied by screening of tendon-related genes (collagen type I, III, decorin, tenascin C and scleraxis) and proteins found in tendon extracellular matrix (ECM) (Collagen I, III, and Tenascin C). Despite the fact that GFs did not seem to influence the synthesis of tendon ECM proteins, EGF and bFGF influenced the expression of tendon-related genes in hAFSCs, while EGF and PDGF-BB stimulated the genetic expression in hASCs. Overall results on cellular alignment morphology, immunolocalization and PCR analysis indicated that both stem cell source can be biochemically induced towards tenogenic commitment, validating the potential of hASCs and hAFSCs for tendon regeneration strategies.
Tipoarticle
URIhttp://hdl.handle.net/1822/28412
DOI10.1371/journal.pone.0083734
Versão da editorahttp://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0083734#pone-0083734-g003
Arbitragem científicayes
AcessoopenAccess
Aparece nas coleções:3B’s - Artigos em revistas/Papers in scientific journals

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