Please use this identifier to cite or link to this item:

TitleOn the use of dexamethasone-loaded liposomes to induce the osteogenic differentiation of human mesenchymal stem cells
Author(s)Monteiro, Nelson
Martins, Albino
Ribeiro, Diana Margarida da Costa
Faria, Susana
Fonseca, Nuno A.
Moreira, João N.
Reis, R. L.
Neves, N. M.
KeywordsBioactive agent
Delivery system
stem cell differentiation
Issue date2015
PublisherJohn Wiley and Sons
JournalJournal of Tissue Engineering and Regenerative Medicine
CitationMonteiro, N., Martins, A., Ribeiro, D., Faria, S., Fonseca, N. A., Moreira, J. N., . . . Neves, N. M. (2015). On the use of dexamethasone-loaded liposomes to induce the osteogenic differentiation of human mesenchymal stem cells. Journal of Tissue Engineering and Regenerative Medicine, 9(9), 1056-1066. doi: 10.1002/term.1817
Abstract(s)Stem cells have received considerable attention by the scientific community because of their potential for tissue engineering and regenerative medicine. The most frequently used method to promote their differentiation is supplementation of the in vitro culture medium with growth/differentiation factors (GDFs). The limitations of that strategy caused by the short half-life of GDFs limit its efficacy in vivo and consequently its clinical use. Thus, the development of new concepts that enable the bioactivity and bioavailability of GDFs to be protected, both in vitro and in vivo, is very relevant. Nanoparticle-based drug delivery systems can be injected, protect the GDFs and enable spatiotemporal release kinetics to be controlled. Liposomes are well-established nanodelivery devices presenting significant advantages, viz. a high load-carrying capacity, relative safety and easy production, and a versatile nature in terms of possible formulations and surface functionalization. The main objective of the present study was to optimize the formulation of liposomes to encapsulate dexamethasone (Dex). Our results showed that the optimized Dex-loaded liposomes do not have any cytotoxic effect on human bone marrow-derived mesenchymal stem cells (hBMSCs). More importantly, they were able to promote an earlier induction of differentiation of hBMSCs into the osteogenic lineage, as demonstrated by the expression of osteoblastic markers, both phenotypically and genotypically. We concluded that Dex-loaded liposomes represent a viable nanoparticle strategy with enhanced safety and efficacy for tissue engineering and regenerative medicine.
DescriptionArticle first published online: 7 Oct. 2013
Publisher version
AccessOpen access
Appears in Collections:3B’s - Artigos em revistas/Papers in scientific journals

Files in This Item:
File Description SizeFormat 
17695-Article 1_final.pdf866,65 kBAdobe PDFView/Open

Partilhe no FacebookPartilhe no TwitterPartilhe no DeliciousPartilhe no LinkedInPartilhe no DiggAdicionar ao Google BookmarksPartilhe no MySpacePartilhe no Orkut
Exporte no formato BibTex mendeley Exporte no formato Endnote Adicione ao seu ORCID